:: Volume 1, Issue 4 (Journal of Clinical and Basic Research(JCBR) 2017) ::
jcbr 2017, 1(4): 1-8 Back to browse issues page
Frequency of AmpC β-lactamase Resistance in Escherichia coli Isolates from Urinary Tract Infections in Gorgan, Iran
Mahdi Arab Zoozani , Ezzat Allah Ghaemi , Ailar Jamalli
Abstract:   (298 Views)
Introduction: AmpC β-lactamases are among the most important cephalosporinases. Production of AmpC β-lactamase in microorganisms is often associated with multidrug resistance and limited treatment options. This study aimed to determine antimicrobial susceptibility pattern and frequency of AmpC β-lactamase genes in Escherichia coli strains isolated from urinary tract infections. Materials and Methods: The study included 154 E. coli isolates from urine samples of patients in medical centers of Gorgan, Iran. Cefoxitin-resistance was evaluated by the Kirby-Bauer method.  Cefoxitin-resistant strains were evaluated using cefoxitin disks alone and combined with boronic acid to confirm presence of AmpC β-lactamase. Presence of MOX gene was investigated by polymerase chain reaction. Results: Among the 154 E. coli isolates, 37 (24%) were cefoxitin-resistant, 16 of which were found to be AmpC β-lactamase-positive in the phenotypic test. In addition, the MOX gene was found in two isolates. Conclusions: The frequency of AmpC β-lactamase-producing E. coli and MOX gene in Gorgan is close to the regional and national average. 
Keywords: Escherichia coli, urinary tract infection, AmpC β-lactamase
Full-Text [PDF 970 kb]   (87 Downloads)    
Type of Study: Research | Subject: Microbiology
Received: 2017/12/27 | Accepted: 2017/12/27 | Published: 2017/12/27
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Volume 1, Issue 4 (Journal of Clinical and Basic Research(JCBR) 2017) Back to browse issues page