Volume 1, Issue 4 (Journal of Clinical and Basic Research(JCBR) 2017)                   jcbr 2017, 1(4): 1-8 | Back to browse issues page

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Arab Zoozani M, Ghaemi E A, Jamalli A. Frequency of AmpC β-lactamase Resistance in Escherichia coli Isolates from Urinary Tract Infections in Gorgan, Iran. jcbr. 2017; 1 (4) :1-8
URL: http://jcbr.goums.ac.ir/article-1-96-en.html
1- Department of Microbiology, Faculty of Medical Sciences, Golestan University of Medical Sciences, Gorgan, Iran
2- Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran
Abstract:   (1767 Views)
Introduction: AmpC β-lactamases are among the most important cephalosporinases. Production of AmpC β-lactamase in microorganisms is often associated with multidrug resistance and limited treatment options. This study aimed to determine antimicrobial susceptibility pattern and frequency of AmpC β-lactamase genes in Escherichia coli strains isolated from urinary tract infections. Materials and Methods: The study included 154 E. coli isolates from urine samples of patients in medical centers of Gorgan, Iran. Cefoxitin-resistance was evaluated by the Kirby-Bauer method.  Cefoxitin-resistant strains were evaluated using cefoxitin disks alone and combined with boronic acid to confirm presence of AmpC β-lactamase. Presence of MOX gene was investigated by polymerase chain reaction. Results: Among the 154 E. coli isolates, 37 (24%) were cefoxitin-resistant, 16 of which were found to be AmpC β-lactamase-positive in the phenotypic test. In addition, the MOX gene was found in two isolates. Conclusions: The frequency of AmpC β-lactamase-producing E. coli and MOX gene in Gorgan is close to the regional and national average. 
Full-Text [PDF 970 kb]   (477 Downloads)    
Article Type: Research | Subject: Microbiology
Received: 2017/12/27 | Accepted: 2017/12/27 | Published: 2017/12/27

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