Volume 5, Issue 4 ( Journal of Clinical and Basic Research (JCBR) 2021)                   jcbr 2021, 5(4): 22-30 | Back to browse issues page

XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

jafari-Sales A, Meshinchi P, Shabkhosh A. In Vitro Antibacterial Activity of Ethanolic Extract of Aloe Vera and Silver Nanoparticles on Standard Strains of Some Pathogenic Bacteria. jcbr. 2021; 5 (4) :22-30
URL: http://jcbr.goums.ac.ir/article-1-332-en.html
1- Department of Microbiology School of Basic Sciences, Kazerun Branch, Islamic Azad University, Kazerun, Iran , a.jafari_1392@yahoo.com
2- Department of Chemical Engineering, University of Tabriz, Tabriz, Iran
3- Department of Microbiology School of Basic Sciences, Ahar Branch, Islamic Azad University, Ahar, Iran
Abstract:   (343 Views)
Background and objectives: Today, with the increasing rate of antibiotic resistance, treatment of bacterial infections has become challenging. Therefore, it is essential to find suitable alternative antibacterial compounds. The aim of this study was to investigate in vitro effects of silver nanoparticles and ethanolic extract of Aloe vera alone and combined on standard strains of some pathogenic bacteria.
Methods: After collection and verification of A. vera plants, extraction was performed by the Soxhlet extractor method. Antibacterial effects of ethanolic extract of A. vera and silver nanoparticles on standard strains of Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa were investigated by agar well diffusion and tube dilution methods, respectively.
Results: The ethanolic extract of A. vera and silver nanoparticles had antibacterial effects on the tested bacteria in a dose-dependent manner.  The ethanolic extract of A. vera was more effective against S. aureus and B. cereus compared to gram-negative bacteria. However, silver nanoparticles were more effective against gram-negative bacteria (P. aeruginosa and E. coli). The effect of combination of ethanolic extract of A. vera and silver nanoparticles was much greater than the effect of either alone. This combination showed the greatest and lowest effect on P. aeruginosa and S. aureus, respectively.
Conclusion: For the first time, this study showed that the combination of ethanolic extract of A. vera and silver nanoparticles is effective against potentially pathogenic bacteria. Given the high rate of antibiotic resistance and side effects of conventional antibiotics, it is recommended to identify active compounds of this plant and evaluate the antimicrobial effects of this combination of fungi and other pathogenic bacteria both in vitro and in vivo.
Full-Text [PDF 453 kb]   (237 Downloads) |   |   Full-Text (HTML)  (38 Views)  
Article Type: Research | Subject: Microbiology
Received: 2021/09/11 | Accepted: 2021/11/6 | Published: 2021/12/28

References
1. Adwan G, Abu-Shanab B, Adwan K. Antibacterial activities of some plant extracts alone and in combination with different antimicrobials against multidrug-resistant Pseudomonas aeruginosa strains. Asian Pacific Journal of Tropical Medicine. 2010;3(4):266-9. [View at Publisher] [DOI] [Google Scholar]
2. Jafari-Sales A, Shahniani A, Fathi R, Malekzadeh P, Mobaiyen H, Bonab FR. Evaluation of Antibacterial Activity of Essential Oil of Ziziphora clinopodioides and Achillea wilhelmsii on Antibiotic-resistant Strains of Staphylococcus aureus. Internal Medicine and Medical Investigation Journal. 2017;2(2):49-56. [View at Publisher] [DOI] [Google Scholar]
3. Mobaiyen H, Jafari Sales A, Sayyahi J. Evaluating Antimicrobial Effects of Centaurea Plant's Essential Oil on Pathogenic Bacteria: Staphylococcus Aureus, Staphylococcus Epidermidis, and Escherichia Coli Isolated from Clinical Specimens. Journal of Fasa University of Medical Sciences. 2016;5(4):479-87. [View at Publisher] [Google Scholar]
4. Hussain AI, Anwar F, Sherazi ST, Przybylski R. Chemical composition, antioxidant and antimicrobial activities of basil (Ocimum basilicum) essential oils depends on seasonal variations. Food chemistry. 2008 Jun 1;108(3):986-95. [View at Publisher] [DOI] [PMID] [Google Scholar]
5. Jafari-Sales A, Bagherizadeh Y, Malekzadeh P, Ahmadi B, Bonab F. Evaluation of the Antimicrobial Effects of Essential Oil of Reseda Lutea L. on Pathogenic Bacteria: Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli. Archives of Clinical Microbiology. 2017;8(3):1-6. [View at Publisher] [DOI] [Google Scholar]
6. Sales AJ. Evaluation of antibacterial activity of ethanol extract of Lavandula Stoechas L. plant on antibiotic-resistant strains Of Staphylococcus Aureus. Journal of Current Research in Science. 2014 Nov 1;2(6):641-5 [Google Scholar]
7. Khalid KA, EL-Ghorab AH. The effect of presowing low temperature on essential oil content and chemical composition of Calendula officinalis. Journal of Essential Oil Bearing Plants. 2006;9(1):32-41. [View at Publisher] [DOI] [Google Scholar]
8. Naqvi S, Khan M, Vohora S. Anti-bacterial, anti-fungal and anthelmintic investigations on Indian medicinal plants. Fitoterapia. 1991;62:221-8.
9. Sundar Rao K. Antibacterial activity of some medicinal plants of Papua New Guinea. International journal of pharmacognosy. 1996;34(3):223-5. [View at Publisher] [DOI] [Google Scholar]
10. Vlietinck A, Van Hoof L, Totte J, Lasure A, Berghe DV, Rwangabo P, et al. Screening of hundred Rwandese medicinal plants for antimicrobial and antiviral properties. Journal of ethnopharmacology. 1995;46(1):31-47. [View at Publisher] [DOI] [Google Scholar]
11. Abd-Alrahman SH, Salem-Bekhit MM, Elhalwagy ME. Chemical composition and antimicrobial activity of Ziziphus jujuba seeds extract. Journal of Pure and Applied Microbiology. 2013;7:379-85. [View at Publisher] [Google Scholar]
12. Tanaka M, Misawa E, Ito Y, Habara N, Nomaguchi K, Yamada M, et al. Identification of five phytosterols from Aloe vera gel as anti-diabetic compounds. Biological and Pharmaceutical Bulletin. 2006;29(7):1418-22. [DOI] [PMID] [Google Scholar]
13. Yazdani D, Rezaei M, Kianbakht S, Khosravani S. A Review on Different Aspects of Aloe vera L. Journal of Medicinal Plants. 2006;5(19):1-8. [View at Publisher] [Google Scholar]
14. Mandrioli R, Mercolini L, Ferranti A, Fanali S, Raggi MA. Determination of aloe emodin in Aloe vera extracts and commercial formulations by HPLC with tandem UV absorption and fluorescence detection. Food Chemistry. 2011;126(1):387-93. [View at Publisher] [DOI] [Google Scholar]
15. Gupta VK, Malhotra S. Pharmacological attribute of Aloe vera: Revalidation through experimental and clinical studies. Ayu. 2012;33(2):193. [DOI] [PMID] [PMCID] [Google Scholar]
16. Kambizi LG, Goosen BM, Taylor MB, Afolayan AJ. Anti-viral effects of aqueous extracts of Aloe ferox and Withania somnifera on herpes simplex virus type 1 in cell culture. South African Journal of Science. 2007 Oct;103(9):359-60. [View at Publisher] [Google Scholar]
17. Gao S-H, Zhao G-X, Yang X-D, Xu L-L. Preparation and antimicrobial effect of aromatic, natural and bacteriostatic foot wash with skin care. Zhongguo Zhong yao za zhi= Zhongguo zhongyao zazhi= China journal of Chinese materia medica. 2013;38(12):2023-6. [View at Publisher] [Google Scholar]
18. Lee KH, Kim JH, Lim DS, Kim CH. Anti‐leukaemic and anti‐mutagenic effects of Di (2‐ethylhexyl) phthalate isolated from Aloe vera Linne. Journal of Pharmacy and Pharmacology. 2000;52(5):593-8. [View at Publisher] [DOI] [PMID] [Google Scholar]
19. Herman A, Herman AP, Domagalska BW, Młynarczyk A. Essential oils and herbal extracts as antimicrobial agents in cosmetic emulsion. Indian journal of microbiology. 2013;53(2):232-7. [View at Publisher] [DOI] [PMID] [PMCID] [Google Scholar]
20. Beger RD. A review of applications of metabolomics in cancer. Metabolites. 2013;3(3):552-74. [View at Publisher] [DOI] [PMID] [PMCID] [Google Scholar]
21. Wikler M, Cockerill F, Craig W. Method for Dilution Antimicrobial Test for Bacteria that Grow Aerobically; Approved Standard. 2006. [Google Scholar]
22. Braydich-Stolle L, Hussain S, Schlager JJ, Hofmann M-C. In vitro cytotoxicity of nanoparticles in mammalian germline stem cells. Toxicological sciences. 2005;88(2):412-9. [View at Publisher] [DOI] [PMID] [PMCID] [Google Scholar]
23. Christian P, Von der Kammer F, Baalousha M, Hofmann T. Nanoparticles: structure, properties, preparation and behaviour in environmental media. Ecotoxicology. 2008;17(5):326-43. [View at Publisher] [DOI] [PMID] [Google Scholar]
24. Jafari-Sales A, Hossein-Nezhad P. Antimicrobial effects of Rosmarinus officinalis methanolic extract on Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa in laboratory conditions. Journal of Medicinal and Chemical Sciences. 2020;3(2):103-8. [View at Publisher] [Google Scholar]
25. Jafari-Sales A, Jafari B, Khaneshpour H, Pashazadeh M. Antibacterial Effect of Methanolic Extract of Rosa damascena on Standard Bacteria Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa in Vitro. International Journal of Nature and Life Sciences. 2020;4(1):40-6. [View at Publisher] [Google Scholar]
26. Jafari-Sales A, Jafari B, Sayyahi J, Zohoori-Bonab T. Evaluation of antibacterial activity of ethanolic extract of malva neglecta and althaea officinalis l. On antibiotic-resistant strains of staphylococcus aureus. J Biol Today World. 2015;4(2):58-62. [DOI] [Google Scholar]
27. Jafari Sales A, Shariat A. Synergistic Effects of Silver Nanoparticles with Ethanolic Extract of Eucalyptus globules on Standard Pathogenic Bacteria in Vitro. Tabari Biomedical Student Research Journal. 2020 Sep 10;2(3):13-21. [DOI] [Google Scholar]
28. Olaleye M, Bello-Michael C. Comparative antimicrobial activities of Aloe vera gel and leaf. African journal of biotechnology. 2005;4(12). [View at Publisher] [Google Scholar]
29. Abraham O, Odiba P, Achumu L, Upu O, Yahaya O, Miachi O, et al. Antimicrobial properties of Aloe Vera juice on the growth of Staphylococcus aureus. Journal of Applied Science and the Environment. 2012;3:1-4. [Google Scholar]
30. George D, Bhat SS, Antony B. Comparative evaluation of the antimicrobial efficacy of Aloe vera tooth gel and two popular commercial toothpastes: An in vitro study. Gen Dent. 2009;57(3):238-41. [Google Scholar]
31. Martinez M, Betancourt J, Alonso-Gonzalez N, Jauregui A. Screening of some Cuban medicinal plants for antimicrobial activity. Journal of ethnopharmacology. 1996;52(3):171-4. [View at Publisher] [DOI] [Google Scholar]
32. Goudarzi M, Fazeli M, Azad M, Seyedjavadi SS, Mousavi R. Aloe vera gel: effective therapeutic agent against multidrug-resistant Pseudomonas aeruginosa isolates recovered from burn wound infections. Chemotherapy Research and Practice. 2015;2015. [View at Publisher] [DOI] [PMID] [PMCID] [Google Scholar]
33. Sadrnia M, Arjomandzadegan M. Comparative study on the effects of Aloe vera extract in clinical strains of Staphylococcus aureus, Klebsiella, Staphylococcus epidermidis and Escherichia coli compared to antibiotics of choice. Journal of Arak University of Medical Sciences. 2014;17(6):39-46. [View at Publisher] [Google Scholar]
34. Anderl JN, Franklin MJ, Stewart PS. Role of antibiotic penetration limitation in Klebsiella pneumoniae biofilm resistance to ampicillin and ciprofloxacin. Antimicrobial agents and chemotherapy. 2000;44(7):1818. [View at Publisher] [DOI] [PMID] [PMCID] [Google Scholar]
35. Irshad S, Butt M. In-vitro antibacterial activity of Aloe barbadensis Miller (Aloe vera). 2011;1(60-65). [Google Scholar]
36. Ibrahim M, Srinivas M, Narasu ML. Phytochemical analysis and antimicrobial evaluation of Aloe vera gel against some human and plant pathogens. Asian Journal of Current Chemistry. 2011;1(1):1-11. [Google Scholar]
37. Pandey R, Mishra A. Antibacterial activities of crude extract of Aloe barbadensis to clinically isolated bacterial pathogens. Applied biochemistry and biotechnology. 2010;160(5):1356-61. [View at Publisher] [DOI] [PMID] [Google Scholar]
38. Asadi M, Khosravi-Darani K, Mortazavi A, Hajseyed Javadi N, Azadnia E, Kiani Harchegani A, et al. Antimicrobial effect of silver nanoparticles produced by chemical reduction on Staphylococcus aureus and Escheirchia coli. Iranian Journal of Nutrition Sciences & Food Technology. 2014;8(4):83-92. [View at Publisher] [Google Scholar]
39. Cho K-H, Park J-E, Osaka T, Park S-G. The study of antimicrobial activity and preservative effects of nanosilver ingredient. Electrochimica Acta. 2005;51(5):956-60. [View at Publisher] [DOI] [Google Scholar]
40. Kim J-S. Antibacterial activity of Ag+ ion-containing silver nanoparticles prepared using the alcohol reduction method. Journal of Industrial and Engineering Chemistry. 2007;13(5):718-22. [View at Publisher] [Google Scholar]
41. Feng QL, Wu J, Chen GQ, Cui F, Kim T, Kim J. A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. Journal of biomedical materials research. 2000;52(4):662-8. [View at Publisher] [DOI] [Google Scholar]
42. Pal S, Tak YK, Song JM. Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the gram-negative bacterium Escherichia coli. Applied and environmental microbiology. 2007;73(6):1712. [View at Publisher] [DOI] [PMID] [PMCID] [Google Scholar]
43. Ruparelia J, Duttagupta S, Chatterjee A, Mukherji S, editors. A comparative study on disinfection potential of nanosilver and nanonickel. Technical poster. Proceedings of the 9th Annual Conference of the Indian Environmental Association (Envirovision-2006), entitled ''Advances in Environmental Management and Technology", Goa, India; 2006. [Google Scholar]
44. Klasen H. A historical review of the use of silver in the treatment of burns. II. Renewed interest for silver. Burns. 2000;26(2):131-8. [View at Publisher] [DOI] [Google Scholar]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2022 CC BY-NC 4.0 | Journal of Clinical and Basic Research

Designed & Developed by : Yektaweb

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0).