Volume 7, Issue 3 ( Journal of Clinical and Basic Research (JCBR) 2023)
jcbr 2023, 7(3): 1-6 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Ehigiator B, Loveth Iyanyi U, Mobisson K S, Ukata O. In vivo and in silico investigation of effects of ethanol extract of moringa oleifera leaves on female fertility, using fruit flies and molecular docking. jcbr 2023; 7 (3) :1-6
URL: http://jcbr.goums.ac.ir/article-1-394-en.html
URL: http://jcbr.goums.ac.ir/article-1-394-en.html
1- Department of Pharmacology and Toxicology, Faculty of Pharmacy Sciences, Madonna University, Elele, Rivers State, Nigeria
2- Department of Pharmacology and Toxicology, Faculty of Pharmacy Sciences, Madonna University, Elele, Rivers State, Nigeria , uchechukwuiyanyi@yahoo.com
3- Department of Human Physiology, Faculty of Basic Medical Sciences, Madonna University, Elele, Rivers State, Nigeria
2- Department of Pharmacology and Toxicology, Faculty of Pharmacy Sciences, Madonna University, Elele, Rivers State, Nigeria , uchechukwuiyanyi@yahoo.com
3- Department of Human Physiology, Faculty of Basic Medical Sciences, Madonna University, Elele, Rivers State, Nigeria
Abstract: (619 Views)
Background: Infertility is an important issue for couples that may cause various psychological and emotional problems. Female infertility disorders play a major role in approximately 50-80% of the causes of infertility in various areas in Nigeria. Moringa oleifera has been proposed as a plant with female fertility enhancement effects. The objective of this study was to assess the fertility-improving effects of ethanol extract of M. oleifera leaf and to determine the phytochemical components causing these effects by in silico analyses.
Methods: The in vitro effects on fertility were evaluated using Drosophila melanogaster (fruit fly) because of its genetic similarities to humans. The copulation duration, mating latency, and the number of emergences from the fruit fly after mating were determined. Three doses (0.025%, 0.05%, and 0.1% w/w) of the M. oleifera ethanol extract were administered to three different groups, while a control group only received feed mixed with ethanol. For in silico studies, 62 compounds were obtained from the PubChem library by mining compounds from articles related to M. oleifera. Next, a ligand library was generated and docked against various targets of interest (estrogen, progesterone, kisspeptin, liver X, PPARG, and 15-PGDH receptors as well as 17β hydroxysteroid dehydrogenase and insulin-degrading enzymes) which have female fertility-enhancing effects.
Results: The in vivo experiments showed that M. oleifera had no effect on copulation duration and mating latency, but interestingly, it enhanced the fertility/emergence of the treated fruit flies. In silico studies suggested that phytochemicals such as rutin, marumoside B, myricetin, and quercetin showed docking scores that may well support previous works on M. oleifera enhancement of female fertility.
Conclusion: The results showed that M. oleifera can enhance fertility in female fruit flies.
Methods: The in vitro effects on fertility were evaluated using Drosophila melanogaster (fruit fly) because of its genetic similarities to humans. The copulation duration, mating latency, and the number of emergences from the fruit fly after mating were determined. Three doses (0.025%, 0.05%, and 0.1% w/w) of the M. oleifera ethanol extract were administered to three different groups, while a control group only received feed mixed with ethanol. For in silico studies, 62 compounds were obtained from the PubChem library by mining compounds from articles related to M. oleifera. Next, a ligand library was generated and docked against various targets of interest (estrogen, progesterone, kisspeptin, liver X, PPARG, and 15-PGDH receptors as well as 17β hydroxysteroid dehydrogenase and insulin-degrading enzymes) which have female fertility-enhancing effects.
Results: The in vivo experiments showed that M. oleifera had no effect on copulation duration and mating latency, but interestingly, it enhanced the fertility/emergence of the treated fruit flies. In silico studies suggested that phytochemicals such as rutin, marumoside B, myricetin, and quercetin showed docking scores that may well support previous works on M. oleifera enhancement of female fertility.
Conclusion: The results showed that M. oleifera can enhance fertility in female fruit flies.
Keywords: Moringa oleifera, fertility Agents, female, Drosophila melanogaster, 3 (or 17)-beta-hydroxysteroid dehydrogenase, In-silico studies (In silico Model)
Article Type: Research |
Subject:
Basic medical sciences
References
1. Zegers-Hochschid F, Adamson GD, De Mouzon J, Ishihara D, Mansour R, Nygren K, et al. The International Committee for Monitoring Assisted Reproductive Technology (ICMART) and the World Health Organization (WHO) revised glossary on ART terminology,2009. Hum Reprod. 2009;24(11):2683-7. [View at Publisher] [DOI] [PMID] [Google Scholar]
2. Agarwal A, Mulgund A, Hamada A, Chyatte MR. A unique view on male infertility around the globe. Reprod Biol Endocrinol.2015;13:37-45. [View at Publisher] [DOI] [PMID] [Google Scholar]
3. Larsen SH, Wagner G, Heitmann L. Sexual function and obesity. Int J Obes. 2007;31(8):1189-98. [View at Publisher] [DOI] [PMID] [Google Scholar]
4. Mukhitdinovna JD. Nay Peritoneal Type of Female Infertility (Mkb-10/N 97.1). Texas J Med Sci. 2022;7:77-8. [View at Publisher] [Google Scholar]
5. Hruska KS, Furth PA, Seifer DB, Sharara FF, Flaws JA. Environmental factors in infertility. Clin Obstet Gynecol. 2000;43(4):821-9. [View at Publisher] [DOI] [PMID] [Google Scholar]
6. Imani B, Eijkemans MJ, Velde ER, Habbema JD, Fauser BC. J Clin Endocrinol Metab. Predictors of patients remaining anovulatory during clomiphene citrate induction of ovulation in normogonadotropic oligoamenorrheic infertility. 1998;83(7):2361-5. [View at Publisher] [DOI] [PMID] [Google Scholar]
7. American Society for Reproductive Medicine. Revised American Society for Reproductive Medicine classification of endometriosis:1996. Fertility sterility. 1997;67(5):817-21. [View at Publisher] [DOI] [PMID] [Google Scholar]
8. Vinodini N, Chatterjee PK, Amemarsoofi A, Suman V, Pai SR. Evaluation of Liver function with Moringa oleifera leaf extract in Cadmium-induced adult Wister albino rats. Int J plants Animal Environ Sci. 2014;4(3):103-6. [View at Publisher] [DOI] [Google Scholar]
9. Demiray S, Pintado ME, Castro PML. Evaluation of phenolic activities of Turkish medicinal plants; Tilia argentea, crataegi folium leaves, and Polygonum bistoria roots. World Acad of Sci Eng and Tech. 2009;3(6):74-9. [View at Publisher] [DOI] [PMID] [Google Scholar]
10. Ekor M. The growing use of herbal medicines: issues relating to adverse reactions and challenge in monitoring safety. Front Pharmacol. 2014;4:177. [View at Publisher] [DOI] [PMID] [Google Scholar]
11. Cajuday LA, Pocsidio GL. Effects of Moringa oleifera Lam on the reproduction of mice. J of Med Plants Res. 2010;4(12):1115-21. [View at Publisher] [DOI] [Google Scholar]
12. Bose CK. Possible role of Moringa oleifera Lam. Root in the Epithelia ovarian cancer. Med Gen Med. 2007;9(1):26-32. [View at Publisher] [PMID] [Google Scholar]
13. Choi JH, Choi KC, Auersperg N, Leung PC. Overexpression of Follicle- Stimulating hormone receptors activates oncogenic pathways in preneoplastic ovarian surface epithelial cells. J Clin Endocrinol Metab. 2004;89(11):5508-16. [View at Publisher] [DOI] [PMID] [Google Scholar]
14. Shukla S, Mathur R, Prakash AO. Biochemical and Physiological alterations in female reproductive organs of cyclic rats treated with aqueous extract of Moringa oleifera Lam. Acta Eur Fertil. 1988;19(4):225-32. [View at Publisher] [PMID] [Google Scholar]
15. Stopper H, Schmitt E, Kobras K. Genotoxicity of Phytoestrogens. Mutation Research Foundation and Molecular Mechanism of mutagenesis. 2005;574(1-2):139-55. [View at Publisher] [DOI] [PMID] [Google Scholar]
16. Ogunsola OA, Owolabi JO, Fabiyi OS, Nwobi NL, Faluyi B, Akinbola AS. Moringa plant parts consumption had effects on reproductive functions in male and female rat models. J Dent Med Sci. 2017;16(10):82-6. [View at Publisher] [DOI] [Google Scholar]
17. Kohler RE. Lords of the Fly: Drosophila genetics and the experimental life. America:University of Chicago Press;1999. P23-9. [View at Publisher] [Google Scholar]
18. Mobisson SK, Obembe AO, Ukoh IE, Duru GO. The Role of Hypothalamic Pituitary Gonadal Axis in Aqueous Extract of Cannabis Sativa Induced Male Reproductive Dysfunction of Albino Wistar Rats. Eur J Pharm and Med Res. 2018;5(1):71-8. [View at Publisher] [Google Scholar]
19. Ehigiator BE, Ozolua RI, Egbogu RO. Evaluation of the sex enhancement and fertility properties of Waltheria indica ethanol root extract in Drosophila melanogaster (Fruit flies). Int J of Pharm and Pharm Res. 2021;3(1):4-8. [View at Publisher] [DOI] [Google Scholar]
20. Brooks WH, Daniel KG, Sung SS, Guida WC. Computational validation of the importance of absolute stereochemistry in virtual screening. J Chem Inf Model. 2008;48(3):639-45. [View at Publisher] [DOI] [PMID] [Google Scholar]
21. Berman HM, Westbrook J, Feng Z, Gilliland G, Bhat TN, Weissig H, et al. The Protein data bank. Nucleic Acids Res. 2000;28(1):235-42. [View at Publisher] [DOI] [PMID] [Google Scholar]
22. Sastry GM, Adzhigrey M, Day T, Annabhimoju R, Sherman W. Protein and Ligand preparation: Parameters, protocols, and influence on virtual screening enrichments. J Comput Aided Mol Des. 2013;27(3):221-34. [View at Publisher] [DOI] [PMID] [Google Scholar]
23. Ehigiator BE, Umana IK, Ikem CJ, Izuegbu C, Ojesebholo RO, Fokunang C. Honey: A remedy for depression. An investigation by experimentalvalidation and molecular docking studies. Int J Pharm and Pharmaceut Sci. 2021:3(1):5-15. [View at Publisher] [DOI] [Google Scholar]
24. Al- Humesh M, Mahmood AY, Lateef RH. Effects of watery extracts from the root of Salvadora persia on the concentration of the sexual hormones and a number of fertility parameters of white mice females. J Tikrit Uni Agric Sci. 2012;12(3):62-8. [View at Publisher] [Google Scholar]
25. Leone A, Fiorillo G, Criscuoli F, Ravasenghi S, Santogostini L, Fico G, et al. Nutritional characterization of phenolic profiling of M. oleifera leaves grown in Chad, Sahrawi refugee camps and Haiti. Int J Mol Sci. 2015;16(8):18923-37. [View at Publisher] [DOI] [PMID] [Google Scholar]
26. Vongsak B, Sithisarn P, Gristsanapan W. Simultaneous HLPC Quantitative analysis of active compounds in leaves of M. oleifera Lam. J Chromatogr Sci. 2014;52(7):641-5. [View at Publisher] [DOI] [PMID] [Google Scholar]
27. Atawodi S, Atawodi J, Idakwo G, Pfundstein B, Haubner R, Wurtele G, et al. Evaluation of the polyphenol content and antioxidant properties of methanolic extracts of the leaves, stem, and root barks of Moringa oleifera Lam. J Med Food. 2010;13(3):710-6. [View at Publisher] [DOI] [PMID] [Google Scholar]
28. El-Alfy T, Essat S, Hegazy A, Amer A, Kamel G. Isolation of biologically active constituents of Moringa oleifera (Moringaceae) growing in Egypt. Pharmacogn Mag. 2011;7(26):109-15. [View at Publisher] [DOI] [PMID] [Google Scholar]
29. Muhammad A, Arulselvan P, Cheah P, Abas F, Fakurazi S. Evaluation of the wound healing properties of the bioactive aqueous extract of M. oleifera Lam on an experimentally induced diabetic animal model. Drug Des Devel Ther. 2016;10:1715-30. [View at Publisher] [DOI] [PMID] [Google Scholar]
30. Nibret E, Wink M. Trypanocidal and antileukemic effects of the essential oils of Hagenia abyssinica, Leonotis ocymifolia, Moringa stenopetala, and their main individual constituents. Phytomedicine. 2010;17(12):911-20. [View at Publisher] [DOI] [PMID] [Google Scholar]
31. Saini R, Sivanesan I, Keum Y. Phytochemicals of M. oleifera: a review of their nutritional, therapeutic and industrial significance. 3 Biotech. 2016;6(2):203. [View at Publisher] [DOI] [PMID] [Google Scholar]
32. Verma A, Vijayakumar M, Mathela C, Rao C. In vitro and in vivo antioxidants properties of different fractions of Moringa oleifera leaves. Food Chem Toxicol. 2009;47(9): 2196-201. [View at Publisher] [DOI] [PMID] []
33. Abd El, Baky H, ElBaroty G. Characterization of Egyptians M. seed oil and its bioactivities. Int J Manag Sci and Bus Res. 2013;2(7):98-108. [View at Publisher] [Google Scholar]
34. Singh BN, Singh BR, Singh RL, Prakash D, Dhakarey R, Upadhyay G. Oxidative DNA damage protective activity, antioxidant and anti quorum sensing potentials of M. oleifera. Food Chem Toxicol. 2009;47(6):1109-16. [View at Publisher] [DOI] [PMID] [Google Scholar]
35. Al- Asmari A, Albalawi S, Athar M, Khan A, Al-Shahrani H, Islam M. Moringa oleifera as an anti-cancer agent against breast and colorectal cancer cell lines. PloS One. 2015;10(8):e01135814. [View at Publisher] [DOI] [PMID] [Google Scholar]
36. Shanker K, Gupta M, Srivastava S, Bawankule D, Pal A, Khanuja S. Determination of active nitrile glycosides in Moringa oleifera by reverse phase HPLC. Food Chem. 2007;105(1):376-82. [View at Publisher] [DOI] [Google Scholar]
37. Sahakitpichan P, Mahidol C, Disadee W, Ruchirawat S, Kanchanapoom T. Unusual glycosides of pyrrole alkaloid and 4′-hydroxyphenylethanamide from leaves of M. oleifera. Phytochemistry. 2011;72(8):791-5. [View at Publisher] [DOI] [PMID] [Google Scholar]
38. Guevara A, Vargas C, Sakurai H, Fujiwara Y, Hashimoto K, Maoko T, et al. An antitumor promoter from Moringa oleifera Lam. Mutat Res. 1999;440(2):181-8. [View at Publisher] [DOI] [PMID] [Google Scholar]
39. Murakami A, Kitazono Y, Jiwajinda S, Koshimizu K, Ohigashi H. Niaziminin, a thiocarbamate from the leaves of Moringa oleifera, holds a strict structural requirement for inhibition of tumor-promoter-induced Epstein- Barr Virus activation. Planta Med. 1998;64(4):319-23. [View at Publisher] [DOI] [PMID] [Google Scholar]
40. Kurzer MS, Xu X. Dietary Phytoestrogens. Annu Rev Nutr. 1997;17:353-81. [View at Publisher] [DOI] [PMID] [Google Scholar]
41. Ehigiator BE, Cobhams AE, Adikwu E, Ben IO. Predicting the Possible Mechanism of Aphrodisiac Action of Pheonix Dactylifera L. J Trop Dis. 2022;10(2):312-320. [View at Publisher] [DOI] [Google Scholar]
42. Herbison AE. Multimodal influence of estrogen upon gonadotropin-releasing hormone neurons. Endocr Rev. 1998;19(3):302-30. [View at Publisher] [DOI] [PMID] [Google Scholar]
43. Konig B, Rauer C, Rosenbaum S, Brandsch C, Eder K, Stangl G. Fasting upregulates PPARα target genes in the brain and influences pituitary hormone expression in PPARα dependent manner. PPAR Res. 2009;2009:9-12. [View at Publisher] [DOI] [PMID] [Google Scholar]
44. Abe M, Hojo T, Kozai K, Okuda K. Possible role of insulin-like factor 3 in bovine corpus luteum. J Vet Med sci. 2013;75(5):629-32. [View at Publisher] [DOI] [PMID] [Google Scholar]
45. Kawamura K, Kumagai J, Sudo S, Chun SY, Pisarska M, Morita H, et al. Paracrine regulation of mammalian oocyte maturation and male germ cell survival. Proc Natl Acad Sci USA. 2004;101(19):7323-8. [View at Publisher] [DOI] [PMID] [Google Scholar]
46. Sugimoto Y, Inazumi T, Tsuchiya S. Roles of prostaglandin receptors in female reproduction. J Biochem. 2015;157(2):73-80. [View at Publisher] [DOI] [PMID] [Google Scholar]
47. Grummer RR, Carroll DJ. A review of lipoprotein cholesterol metabolism: Importance to ovarian function. J Anim Sci. 1998;66(12):3160-73. [View at Publisher] [DOI] [PMID] [Google Scholar]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0).