en علوم پایه پزشکی Basic medical sciences پژوهشي Research <span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:115%"><a name="_Hlk117843170"></a></span></span><span style="font-size:12px;"><b>Background:</b> Infertility is an important issue for couples that may cause various psychological and emotional problems. Female infertility disorders play a major role in approximately 50-80% of the causes of infertility in various areas in Nigeria. <i>Moringa oleifera</i> has been proposed as a plant with female fertility enhancement effects. The objective of this study was to assess the fertility-improving effects of ethanol extract of <i>M. oleifera</i> leaf and to determine the phytochemical components causing these effects by in silico analyses.<b><span style="font-size:8.0pt"></span></b><br> <b>Methods:</b> The in vitro effects on fertility were evaluated using <i>Drosophila melanogaster </i>(fruit fly) because of its genetic similarities to humans. The copulation duration, mating latency, and the number of emergences from the fruit fly after mating were determined. Three doses (0.025%, 0.05%, and 0.1% w/w) of the <i>M. oleifera</i> ethanol extract were administered to three different groups, while a control group only received feed mixed with ethanol.<i> </i>For in silico studies, 62 compounds were obtained from the PubChem library by mining compounds from articles related to <i>M.<i> </i>oleifera</i>. Next, a ligand library was generated and docked against various targets of interest (estrogen, progesterone, kisspeptin, liver X, PPARG, and 15-PGDH receptors as well as 17&beta; hydroxysteroid dehydrogenase and insulin-degrading enzymes) which have female fertility-enhancing effects.<br> <b>Results:</b> The in vivo experiments showed that <i>M. oleifera </i>had no effect on copulation duration and mating latency, but interestingly, it enhanced the fertility/emergence of the treated fruit flies. In silico studies suggested that phytochemicals such as rutin, marumoside B, myricetin, and quercetin showed docking scores that may well support previous works on <i>M. oleifera</i> enhancement of female fertility.<br> <b>Conclusion</b>: The results showed that<b> </b><i>M. oleifera </i>can enhance fertility in female fruit flies.<span style="font-size:8.0pt"></span></span></span> Moringa oleifera,fertility Agents, female,Drosophila melanogaster,3 (or 17)-beta-hydroxysteroid dehydrogenase,In-silico studies (In silico Model) 1 6 http://jcbr.goums.ac.ir/browse.php?a_code=A-10-409-1&slc_lang=en&sid=1 Ben Ehigiator beevee8488@gmail.com 10031947532846004734 10031947532846004734 No Department of Pharmacology and Toxicology, Faculty of Pharmacy Sciences, Madonna University, Elele, Rivers State, Nigeria Uchechukwu Loveth Iyanyi uchechukwuiyanyi@yahoo.com 10031947532846004735 10031947532846004735 Yes Department of Pharmacology and Toxicology, Faculty of Pharmacy Sciences, Madonna University, Elele, Rivers State, Nigeria Kelechi Samuel Mobisson samuelmobisson@gmail.com 10031947532846004736 10031947532846004736 No Department of Human Physiology, Faculty of Basic Medical Sciences, Madonna University, Elele, Rivers State, Nigeria Onuchukwu Ukata onuchukwuukata@gmail.com 10031947532846004737 0009-0003-6696-5366 No Department of Pharmacology and Toxicology, Faculty of Pharmacy Sciences, Madonna University, Elele, Rivers State, Nigeria