دوره 9، شماره 3 - ( 7-1404 )
جلد 9 شماره 3 صفحات 26-22 |
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Jafari-Sales A, Golestani A, Ghahremani Z, Pashazadeh M. Evaluation of the antibacterial properties of methanolic pomegranate peel extract against standard strains of Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa, and Escherichia coli: An in Vitro Study. jcbr 2025; 9 (3) :22-26
URL: http://jcbr.goums.ac.ir/article-1-532-fa.html
URL: http://jcbr.goums.ac.ir/article-1-532-fa.html
Evaluation of the antibacterial properties of methanolic pomegranate peel extract against standard strains of Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa, and Escherichia coli: An in Vitro Study. Journal of Clinical and Basic Research. 1404; 9 (3) :22-26
چکیده: (330 مشاهده)
Background: The increasing prevalence of antimicrobial resistance has intensified the search for effective natural alternatives to synthetic antibiotics. Pomegranate (Punica granatum) peel, a widely available agricultural by-product, is known for its rich phytochemical composition. We aimed to quantify the in vitro antibacterial activity of pomegranate peel methanolic extract (PPME) against selected Gram-positive and Gram-negative bacteria and analyze their differential susceptibility.
Methods: Here, PPME was prepared using a Soxhlet apparatus, and its antibacterial efficacy was tested against Staphylococcus aureus, Bacillus cereus, Escherichia coli, and Pseudomonas aeruginosa via the agar well-diffusion method at the concentrations of 50, 100, 200, and 400 mg/mL. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined using the broth dilution technique. Data were analyzed employing one-way ANOVA.
Results: The PPME demonstrated notable dose-dependent antibacterial activity (p < 0.0001), especially against Gram-negative bacteria. Escherichia coli was the most sensitive bacterial strain, with the largest inhibition zone (27 mm) at 400 mg/mL and the lowest MIC (12.5 mg/mL) and MBC (25 mg/mL). Gram-positive bacteria were more resistant to PPME, for which Bacillus cereus showed the highest MIC (100 mg/mL) and MBC (200 mg/mL).
Conclusion: Pomegranate peel extract possesses potent antibacterial activity, exhibiting relatively stronger efficacy against Gram-negative pathogens, offering it a promising sustainable candidate for developing novel antibacterial agents, especially against resistant Gram-negative pathogenic strains. Further research is warranted to identify the active compounds of PPME and their mechanisms of action.
Methods: Here, PPME was prepared using a Soxhlet apparatus, and its antibacterial efficacy was tested against Staphylococcus aureus, Bacillus cereus, Escherichia coli, and Pseudomonas aeruginosa via the agar well-diffusion method at the concentrations of 50, 100, 200, and 400 mg/mL. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined using the broth dilution technique. Data were analyzed employing one-way ANOVA.
Results: The PPME demonstrated notable dose-dependent antibacterial activity (p < 0.0001), especially against Gram-negative bacteria. Escherichia coli was the most sensitive bacterial strain, with the largest inhibition zone (27 mm) at 400 mg/mL and the lowest MIC (12.5 mg/mL) and MBC (25 mg/mL). Gram-positive bacteria were more resistant to PPME, for which Bacillus cereus showed the highest MIC (100 mg/mL) and MBC (200 mg/mL).
Conclusion: Pomegranate peel extract possesses potent antibacterial activity, exhibiting relatively stronger efficacy against Gram-negative pathogens, offering it a promising sustainable candidate for developing novel antibacterial agents, especially against resistant Gram-negative pathogenic strains. Further research is warranted to identify the active compounds of PPME and their mechanisms of action.
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