Volume 8, Issue 2 (Journal of Clinical and Basic Research (JCBR) 2024)
jcbr 2024, 8(2): 24-27 |
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Ali Baqher Hussaini S, Manisha Goud P, Karamchandu S, Manoja V, . A, Suneetha A. Expression of MLH-1, PMS-2, MSH-2, MSH-6 markers and histopathological evaluation in colorectal cancer. jcbr 2024; 8 (2) :24-27
URL: http://jcbr.goums.ac.ir/article-1-449-en.html
URL: http://jcbr.goums.ac.ir/article-1-449-en.html
Syed Ali Baqher Hussaini1 
, Punjalan Manisha Goud2 , Shilpa Karamchandu2 , Vaddadi Manoja * 3, Amal .2 , Angara Suneetha2
, Punjalan Manisha Goud2 , Shilpa Karamchandu2 , Vaddadi Manoja * 3, Amal .2 , Angara Suneetha2
1- SN Diagnostic Centre, Kalaburagi, Karnataka, India
2- Department of Pathology, SVS Medical College, Yenugonda, Mahabubnagar, Telangana-509001, India
3- Department of Pathology, SVS Medical College, Yenugonda, Mahabubnagar, Telangana-509001, India ,vali_shaik31@rediffmail.com
2- Department of Pathology, SVS Medical College, Yenugonda, Mahabubnagar, Telangana-509001, India
3- Department of Pathology, SVS Medical College, Yenugonda, Mahabubnagar, Telangana-509001, India ,
Abstract: (262 Views)
Background: The clinicopathological characterization of biomarkers to identify microsatellite instability in patients with colon cancers has not been fully studied. This study aims to evaluate the spectrum of histopathology of MLH1, MSH2, MSH6, and PMS2 markers to identify microsatellite instability in patients with colon cancers.
Methods: The study analyzed the association between clinicopathological features and immunohistochemistry MLH-1, MSH-2, PMS-2, & MSH-6 expressions in 36 resected colorectal cancer samples. Patients' data were collected retrospectively, including tumor localization, size, the origin of polyp, mucinous differentiation, tumor stage, lymphovascular and perineural invasion, surgical boundary, and lymph node metastases. The primary antibodies, such as MLH-1, MSH-2, PMS-2, & MSH-6, were tested immunohistochemically.
Results: The number of male cases was higher than female cases, 63.89% vs. 36.11%. The highest incidence (27.78%) of colorectal cancer was seen in the age group of 51-60 years. Tumors were predominantly adenocarcinoma NOS (69.44%). Moderately differentiated tumors (55.56%) were higher in number than poorly differentiated and well-differentiated ones. The highest stage seen was stage III in 50%. 80.56% of cases showed perineural invasion, 27.78% showed lymphovascular invasion, and 52.78% showed nodal metastasis. Right colon placement was associated with mucinous adenocarcinoma and a mucinous component. The ulcerous tumors were quite small, and their pathological tumor stage was higher. Tumors developing from polyps were large in size and had a lower pathological tumor stage. The study found that deletion of MSH-2 & MSH-6 expressions was substantially associated with right-colon placement and poor differentiation. In poorly differentiated adenocarcinomas, mucinous, lymphovascular, and perineural invasions were common. Tumors with mucinous differentiation showed less lymphovascular invasion and lymph node metastases. Perineural invasion was not noted in the pT1 or pT2 tumor stages.
Conclusion: A strong correlation was found between immunohistochemical markers and clinical characteristics of tumors with microsatellite instability. However, these conclusions should be supported by large-scale investigations involving molecular PCR and other approaches.
Methods: The study analyzed the association between clinicopathological features and immunohistochemistry MLH-1, MSH-2, PMS-2, & MSH-6 expressions in 36 resected colorectal cancer samples. Patients' data were collected retrospectively, including tumor localization, size, the origin of polyp, mucinous differentiation, tumor stage, lymphovascular and perineural invasion, surgical boundary, and lymph node metastases. The primary antibodies, such as MLH-1, MSH-2, PMS-2, & MSH-6, were tested immunohistochemically.
Results: The number of male cases was higher than female cases, 63.89% vs. 36.11%. The highest incidence (27.78%) of colorectal cancer was seen in the age group of 51-60 years. Tumors were predominantly adenocarcinoma NOS (69.44%). Moderately differentiated tumors (55.56%) were higher in number than poorly differentiated and well-differentiated ones. The highest stage seen was stage III in 50%. 80.56% of cases showed perineural invasion, 27.78% showed lymphovascular invasion, and 52.78% showed nodal metastasis. Right colon placement was associated with mucinous adenocarcinoma and a mucinous component. The ulcerous tumors were quite small, and their pathological tumor stage was higher. Tumors developing from polyps were large in size and had a lower pathological tumor stage. The study found that deletion of MSH-2 & MSH-6 expressions was substantially associated with right-colon placement and poor differentiation. In poorly differentiated adenocarcinomas, mucinous, lymphovascular, and perineural invasions were common. Tumors with mucinous differentiation showed less lymphovascular invasion and lymph node metastases. Perineural invasion was not noted in the pT1 or pT2 tumor stages.
Conclusion: A strong correlation was found between immunohistochemical markers and clinical characteristics of tumors with microsatellite instability. However, these conclusions should be supported by large-scale investigations involving molecular PCR and other approaches.
Keywords: Colorectal neoplasms, Microsatellite instability, Immunohistochemistry, DNA Mismatch Repair
Article Type: Research |
Subject:
Basic medical sciences
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