Ameer Abdulraheem Handhal1 
, Mahin Gholipur2 , Fatima Mohammad zadeh2 , Somayeh Ghorbani3 , Safoura Khajeniazi * 4
, Mahin Gholipur2 , Fatima Mohammad zadeh2 , Somayeh Ghorbani3 , Safoura Khajeniazi * 4
1- Department of Clinical Biochemistry, School of Medical Sciences, Golestan University of Medical Sciences, Gorgan,Iran
2- Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran
3- Cancer Research Center, Golestan University of Medical Sciences, Gorgan, Iran
4- Department of Clinical Biochemistry, School of Medical Sciences, Golestan University of Medical Sciences, Gorgan,Iran; Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran ,niazie80@gmail.com
2- Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran
3- Cancer Research Center, Golestan University of Medical Sciences, Gorgan, Iran
4- Department of Clinical Biochemistry, School of Medical Sciences, Golestan University of Medical Sciences, Gorgan,Iran; Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran ,
Abstract: (79 Views)
Background: Hypothyroidism is a significant contributor to complications in various metabolic and cardiovascular diseases. Thyroid hormones, including lipids, play a crucial role in macromolecule metabolism. C-reactive protein (CRP) is a key marker for cardiovascular diseases and hypothyroidism independently. This study aimed to evaluate serum high-sensitivity CRP (hs-CRP) levels, lipid profiles, and their relationship with thyroid hormones in patients with hypothyroidism compared to a control group.
Methods: This case-control study included 90 participants (45 hypothyroid patients and 45 healthy controls). Blood samples were collected, centrifuged to separate serum, and analyzed using commercial kits. ELISA measured T3, T4, TSH, and hs-CRP, while TG, HDL, LDL, VLDL, and total cholesterol were measured by spectroscopy.
Results: The findings revealed higher hs-CRP levels in hypothyroid patients compared to controls, although not statistically significant (P-value = 0.09). Hs-CRP positively correlated with TSH, T3, and TG but negatively correlated with T4, total cholesterol, VLDL, and HDL in hypothyroid subjects. These correlations were only significant between hs-CRP and TG and hs-CRP and LDL (P-value < 0.05).
Conclusion: The positive correlation between hs-CRP and TSH in hypothyroidism highlights a potential link between hypothyroidism and cardiovascular disease.
Methods: This case-control study included 90 participants (45 hypothyroid patients and 45 healthy controls). Blood samples were collected, centrifuged to separate serum, and analyzed using commercial kits. ELISA measured T3, T4, TSH, and hs-CRP, while TG, HDL, LDL, VLDL, and total cholesterol were measured by spectroscopy.
Results: The findings revealed higher hs-CRP levels in hypothyroid patients compared to controls, although not statistically significant (P-value = 0.09). Hs-CRP positively correlated with TSH, T3, and TG but negatively correlated with T4, total cholesterol, VLDL, and HDL in hypothyroid subjects. These correlations were only significant between hs-CRP and TG and hs-CRP and LDL (P-value < 0.05).
Conclusion: The positive correlation between hs-CRP and TSH in hypothyroidism highlights a potential link between hypothyroidism and cardiovascular disease.
Article Type: Research |
Subject:
Biochemistry
References
1. Li T, Geng H, Wang Y, Wu Z, Yang S, Hu Y. Causal Association of Thyroid Signaling with C-Reactive Protein: A Bidirectional Mendelian Randomization. Hindawi Computational and Mathematical Methods in Medicine. 2022;2022:8954606. [View at Publisher] [DOI] [PMID] [Google Scholar]
2. Michalaki MA , Vagenakis AG , Leonardou AS , Argentou MN, Habeos LG, Makri MG, et al. Thyroid function in humans with morbid obesity. Thyroid. 2006;16(1):73-8. [View at Publisher] [DOI] [PMID] [Google Scholar]
3. Biondi B, Cappola AR, Cooper DS. Subclinical hypothyroidism. JAMA. 2019;322(2):153-60. [View at Publisher] [DOI] [PMID] [Google Scholar]
4. Rizos CV, Elisaf MS, Liberopoulos EN. Effects of Thyroid Dysfunction on Lipid Profile. Open Cardiovasc Med J. 2011;5:76-84 [View at Publisher] [DOI] [PMID] [Google Scholar]
5. Duntas LH. Thyroid disease and lipids. Thyroid. 2002;12(4):287-93. [View at Publisher] [DOI] [PMID] [Google Scholar]
6. Asvold BO, Vatten LJ, Nilsen TI, Bjoro T. The association between TSH within the reference range and serum lipid concentrations in a population-based study. The HUNT Study. Eur J Endocrinol. 2007;156(2):181-6. [View at Publisher] [DOI] [PMID] [Google Scholar]
7. Ahmad N, Iqbal S, Javed M, Ahmad M, Nafees S, Augusthy A. Relationship of Hypothyroidism with Lipid Levels, hs-CRP and BMI. Int J Cur Res Rev. 2021;13(23):104-7. [View at Publisher] [DOI] [Google Scholar]
8. Jose E, Miranda P, Bittencourt M, Santos I , Lotufo P , Benseñor I. Thyroid Function and High-Sensitivity C-Reactive Protein in Cross-Sectional Results from the Brazilian Longitudinal Study of Adult Health. Eur Thyroid J. 2016;5(4):240-6 [View at Publisher] [DOI] [PMID] [Google Scholar]
9. Janssen IM, Homan J, Schijns W, Betzel B , Aarts E , Berends F, et al. Subclinical hypothyroidism and its relation to obesity in patients before and after Roux-en-Y gastric bypass. Surg Obes Relat Dis. 2015;11(6):1257-63. [View at Publisher] [DOI] [PMID] [Google Scholar]
10. O'Brien T, Dinneen SF, O'Brien PC, Palumbo PJ. Hyperlipidemia in patients with primary and secondary hypothyroidism. Mayo Clin Proc. 1993;68(9):860-6. [View at Publisher] [DOI] [PMID] [Google Scholar]
11. Bensenor IM, Goulart AC, Molina Mdel C, Miranda E , Santos I , Lotufo P. Thyrotropin levels, insulin resistance, and metabolic syndrome: a cross-sectional analysis in the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil). Metab Syndr Relat Disord. 2015;13(8):362-9. [View at Publisher] [DOI] [PMID] [Google Scholar]
12. Albert MA , Danielson E, Rifai N, Ridker PM. Effect of statin therapy on C-reactive protein levels; the pravastatin inflammation/CRP evaluation (PRINCE): a randomized trial and cohort study. JAMA. 2001;286(1):64-70. [View at Publisher] [DOI] [PMID] [Google Scholar]
13. Czarnywojtek A, Owecki M, Zgorzalewicz-Stachowiak M, Woliński K, Szczepanek-Parulska E, Budny B. The Role of Serum C-Reactive Protein Measured by High-Sensitive Method in Thyroid Disease. Arch Immunol Ther Exp. 2014;62:501-9. [View at Publisher] [DOI] [PMID] [Google Scholar]
14. Tuzcu A, Bahceci M, Gokalp D, Tuzun Y, Gunes K. Subclinical Hypothyroidism may be associated with elevated High-sensitive C-reactive protein (low grade inflammation) And fasting hyperinsulinemia. Endocr J. 2005;52(1):89-94. [View at Publisher] [DOI] [PMID] [Google Scholar]
15. Ganesan A, Sethulekshmi S, et al: Relationship between hs-CRP and TSH in patients with subclinical hypothyroidism. Rom J Diabetes Nutr Metab Dis. 2021;28(4):391-4. [View at Publisher] [DOI] [Google Scholar]
16. Ridker PM, Rifai N, Rose L, Buring JE, Cook NR. Comparison of CRP and LDL cholesterol in prediction of first cardiovascular event. N Engl J Med. 2002;347(20):1557-65. [View at Publisher] [DOI] [PMID] [Google Scholar]
17. Sharma P, Prashar N, Sharma G, Singh H, Sharma R. Lipid profile and hs-CRP levels in patients with subclinical hypothyroidism. Int J Med Sci Public Health. 2016;5(6):1233-6. [View at Publisher] [DOI] [Google Scholar]
18. Christ-Crain M, Meier C, Guglielmetti M, Huber PR, Riesen W, Staub JJ, et al. Elevated C-reactive protein and homocytsteine values: cardiovascular risk factors in hypothyroidism? A cross-sectional and double blind, placebo controlled trial. Atherosclerosis. 2003;166(2):379-86. [View at Publisher] [DOI] [PMID] [Google Scholar]
19. Jung CH, Sung KC, Shin HS, Rhee EJ, Lee WY, Kim BS, et al. Thyroid dysfunction to cardiovascular risk factors such as lipid profile, hs-CRP and waist hip ratio in Korea. Korean J Intern Med. 2003;18(3):146-53. [View at Publisher] [DOI] [PMID] [Google Scholar]
20. Shantha G, Kumar A, Jeyachandran V, Rajamanickam D, Rajkumar K, Salim S, et al. Association between primary hypothyroidism and metabolic syndrome and the role of C reactive protein. Thyroid Research. 2009;2(2):1-7. [View at Publisher] [DOI] [PMID] [Google Scholar]
21. Krishnamurthy H, Ravitej S. Predictive Significance of High-Sensitive C-reactive Protein in Subclinical Hypothyroidism. J Clin Diagn Res. 2022;16(10):15-7. [View at Publisher] [DOI] [Google Scholar]
22. Ahmad N, Nagtilak S, Sharma A, Parashar P, Rastogi A. Role of Circulating C-Reactive Protein in Thyroid Disorders. JMSCR. 2018;06(02):485-9. [View at Publisher] [DOI] [Google Scholar]
23. Sharma R, Sharma TK, Kaushik GG, Sharma S, Vardey SK, Sinha M. Subclinical hypothyroidism and its association with cardiovascular risk factors.Clin Lab. 2011;57(9-10):719-24. [View at Publisher] [DOI] [Google Scholar]
24. Ahmad N, Ahmad M, Gupta A, Sharma AK. Association of Elevated Serum hs-CRP Levels with the Development of Cardiovascular Disease in Known Cases of Hypothyroidism: A case-control study. National Journal of Laboratory Medicine. 2022;11(1):16-9. [View at Publisher] [DOI] [Google Scholar]
25. Kshetrimayum V, Usha SMR, Vijayalakshmi P. A study of hs-CRP and lipid profile in hypothyroid adults at tertiary care hospital. International Journal of Clinical Biochemistry and Research. 2019;6(3):303-10. [View at Publisher] [DOI] [Google Scholar]
26. Wang C. The Relationship between Type 2 Diabetes Mellitus and Related Thyroid Diseases. J Diabetes Res. 2013;2013:390534. [View at Publisher] [DOI] [PMID] [Google Scholar]
27. Jublanc C, Bruckert E, Giral P, Chapman MJ, Leenhardt L, Carreau V , et al. Relationship of circulating C-reactive protein levels to thyroid status and cardiovascular risk in hyperlipidemic euthyroid subjects: low free thyroxine is associated with elevated hs-CRP. Atherosclerosis. 2004;172(1):7-11. [View at Publisher] [DOI] [PMID] [Google Scholar]
28. Rustam S, Hassan B. Assessment of inflammatory marker (Hs-CRP and OPN) and other parameters in Hypothyroidism. Teikyo Medical Journal. 2021;44(6):2325-31. [View at Publisher] [Google Scholar]
29. Jha U, et al: To Compare the Thyroid Profile, hs-CRP, and Lipid Profile in Newly Diagnosed Hypothyroid Adults to Controls, as well as to Compare the above Parameters in Subclinical and Clinical Hypothyroid Instances. European Journal of Molecular & Clinical Medicine (EJMCM). 2021; 4: 2515-8260. [Google Scholar]
30. Sumanth Kumar B, Sampson U, Pratheeba J, Ponnazhagan K. Study of Vitamin D, hs-CRP and Lipid Profile Status in Newly Diagnosed. Hypothyroid Patients in the Local Population of Kanchipuram District. Sch Int J Biochem. 2020;3(9):201-5. [View at Publisher] [DOI]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0).