[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 2, Issue 1 ((Journal of Clinical and Basic Research(JCBR) 2018) ::
jcbr 2018, 2(1): 1-5 Back to browse issues page
Evaluation of Antibiotic Resistance and β- lactamase Production in Clinical Isolates from a Tertiary Care Hospital in Central India
Vaibhavi Vijay Nanoty 1, Gopal Nandlal Agrawal * 2, Supriya Sanjay Tankhiwale
1- 1. Department of Microbiology, Government Medical College Akola, Collector Office Road, Near Ashok Vatika, Akola, Maharashtra, India, 1. Department of Microbiology, Government Medical College Akola, Collector Office Road, Near Ashok Vatika, Akola, Maharashtra, India
2- 2. Department of Microbiology, Government Medical College Nagpur, Hanuman Nagar, Nagpur, Maharashtra, India
Abstract:   (466 Views)
Introduction: Antimicrobial resistance in bacterial pathogens is associated with high morbidity and mortality. We aimed to evaluate antibiotic resistance and β-lactamase production in clinical isolates of a tertiary care hospital in Central India. Materials and Methods: Clinical isolates (n=6472 isolates) from patients with infection were identified using standard microbiological techniques. Antibiotic susceptibility testing was performed according to the CLSI guidelines using the Kirby-Bauer disc diffusion technique. AmpC production in Enterobacteriaceae isolates was tested in screening test. Cloxacillin combined disc diffusion test was performed for both inducible and non-inducible AmpC using cefoxitin disc with and without cloxacillin. Metallo-β-lactamase production in Enterobacteriaceae isolates was tested in screening test (positive= zone diameter of < 21 mm for meropenem). Non-fermenting Gram-negative isolates were tested by combined disc test using imipenem and imipenem-EDTA discs. Results: Majority of the bacteremia cases were caused by Staphylococcus aureus (43.13%), non-fermenting spp. (27.44%) and coagulase-negative staphylococci (11.76%). Escherichia coli (55.85%) was the main cause of urinary tract infection followed by Acinetobacter spp. (11.71%) and Klebsiella pneumoniae (10.36%). No resistance to linezolid was seen in Gram-positive isolates. Frequency of vancomycin-resistance was about 9% in Enterococcus spp. Methicillin resistance was seen in 19% of S. aureus isolates. Enterobacteriaceae and Citrobacter freundii isolates were completely resistant to aminopenicillin, first and second-generation cephalosporins and cefamycin. Moreover, Klebsiella isolates were resistant to aminopenicillin. Enterobacteriaceae isolates showed resistance to aminopenicillin (89.87%), cephalosporins (54-90%) and cephamycin (37-45%). E. coli isolates were sensitive to PIT (87-96%) and imipenem (99.68-100%). ESBL production was seen in 166 Enterobacteriaceae isolates (30.24%) in DDST and CPCT. AmpC production was seen in 15 (2.73%) Enterobacteriaceae isolates.  Total β-lactamase production was found in 19.23% of the isolates tested. The frequency of β-lactamase production was highest in K. pneumoniae (51.67%). Co-production of β-lactamases was not observed. Conclusions: It is necessary to closely monitor drug resistance and β-lactamase production. Moreover, it is recommended to perform routine β-lactamase testing in microbiology laboratories for determining prevalence of antibiotic resistance and controlling their spread.
Keywords: Antibiotic Resistance, ESBL, β-lactamase, Enterobacteriaceae
Full-Text [PDF 251 kb]   (132 Downloads)    
Article Type: Research | Subject: Genaral
Received: 2017/12/27 | Accepted: 2017/12/27 | Published: 2017/12/27
* Corresponding Author Address: 2. Department of Microbiology, Government Medical College Nagpur, Hanuman Nagar, Nagpur, Maharashtra, India
Add your comments about this article
Your username or Email:

CAPTCHA code


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Nanoty V V, Agrawal G N, Tankhiwale S S. Evaluation of Antibiotic Resistance and β- lactamase Production in Clinical Isolates from a Tertiary Care Hospital in Central India. jcbr. 2018; 2 (1) :1-5
URL: http://jcbr.goums.ac.ir/article-1-95-en.html


Volume 2, Issue 1 ((Journal of Clinical and Basic Research(JCBR) 2018) Back to browse issues page
Journal of Clinical and Basic Research Journal of Clinical and Basic Research
Persian site map - English site map - Created in 0.06 seconds with 31 queries by YEKTAWEB 3781