Introduction: Intense exercise may cause immunological changes and muscle damage. The aim of this study was to investigate the effect of one session of resistance training with and without blood flow restriction on serum levels of creatine kinase (CK) and lactate dehydrogenase (LDH) in female athletes. Materials and Methods: In this semi-experimental study, 30 female basketball players were randomly divided into a traditional resistance training group (without blood flow restriction), a resistance training group (with blood flow restriction) and a control group. Blood samples were taken before the first session and 30 minutes after the last training session. T-test and one-way analysis of variance were used for evaluation of within group and between group differences, respectively. All statistical analyses were performed in SPSS (version 23) at significance level of 0.05. Results: Serum level of CK increased significantly in all group, while serum LDH increased significantly only in the experimental groups when compared with pre-test levels (P<0.05). There was no significant difference in serum levels of CK and LDH between the experimental groups and the control group. Conclusions: Resistance training with blood flow restriction and high-intensity resistance training without blood flow restriction have similar effects on markers of muscle damage.