en Neuroscience پژوهشي Research <div style="text-align: justify;"><span style="font-size:12px;"><span style="font-family:Times New Roman;"><b>Background:</b> Neuropathic pain results from nerve injury and involves pro-inflammatory cytokines that activate pain-sensing neurons, contributing to both the onset and maintenance of pain. This study investigated the effect of lipopolysaccharide (LPS) preconditioning on pain following sciatic nerve constriction in rats.<br> <b>Methods:</b> Neuropathic pain was induced via the chronic constriction injury (CCI). Rats received 0.1 mg LPS intraperitoneally three days before CCI. They were divided into four groups: control, CCI without LPS, CCI with LPS, and LPS alone. Pain behavior was assessed on days 0, 7, and 14 using acetone and hot plate tests.<br> <b>Results:</b> LPS alone did not affect pain sensitivity. CCI increased responses to thermal and cold stimuli. However, prior LPS reduced these responses compared to CCI alone.<br> <b>Conclusion</b>: This study suggests that 0.1 mg LPS can alleviate neuropathic pain by increasing pain threshold after nerve injury.</span></span></div> Neuropathic pain, Cytokines, Lipopolysaccharides, Chronic constriction injury 14 16 http://jcbr.goums.ac.ir/browse.php?a_code=A-10-235-2&slc_lang=en&sid=1 Soqra Hesam soqra.hesam@gmail.com 10031947532846006555 10031947532846006555 Yes Neuroscience Research Center, Biomedical Research Institute, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran Fateme Piri fateme.piri7272f@gmail.com 10031947532846006556 0009-0005-3542-7551 No Neuroscience Research Center, Biomedical Research Institute, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran Mahshid Moradpour mahshidmp93@gmail.com 10031947532846006557 0009-0007-6964-636x No Neuroscience Research Center, Biomedical Research Institute, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran Hamid Sepehri hamsep49@yahoo.com 10031947532846006558 10031947532846006558 No Neuroscience Research Center, Biomedical Research Institute, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran