en علوم پایه پزشکی Basic medical sciences پژوهشي Research <div style="text-align: justify;"><span style="font-size:12px;"><span style="font-family:Times New Roman;"><b>Background:</b> Spermatogenesis can progress only normally in a tightly regulated redox environment under the fine regulation of testosterone and other hormones. This process is particularly vulnerable to stress, which can disturb the gut microbiome&mdash;an important factor in testicular function. However, it is not known if supplementation with probiotics can mitigate the effects of psychological stress on sperm production. This study aimed to investigate the effect of gut probiotics on testicular function of rats during psychological stress.<br> <b>Methods:</b> Thirty-five adult male rats were randomly assigned into seven groups with five rats each. The research was divided into two phases: one week and two weeks&#39; administration, in which the rats were subjected to probiotics only, stress only, and probiotics plus stress. Data were analyzed using one-way ANOVA and student Newman-Keuls post hoc test for multiple comparisons at p <0.05<br> <b>Results:</b> Chronically stressed reduced testicular weight and testicular relative organ weight, the same as acutely stressed rats. However, treatment with probiotics resulted in statistically significant differences as a mitigating effect.<br> <b>Conclusion</b>: Gut probiotics have a generally beneficial impact on the testes when evaluating biochemically and morphological parameters.</span></span><br> &nbsp;</div> Gut Probiotic, Stress, Psychological, Spermatogenesis, Testis 1 8 http://jcbr.goums.ac.ir/browse.php?a_code=A-10-593-1&slc_lang=en&sid=1 Oluwatoyin Ojuolape Medubi omedubi@unilag.edu.ng 10031947532846006547 10031947532846006547 No Department of Physiology, Faculty of Basic Medical Sciences, University of Lagos, Akoka Lagos, Nigeria Leke Jacob Medubi jmedubi@unilag.edu.ng 10031947532846006548 10031947532846006548 No Department of Anatomy, Faculty of Basic Medical Sciences, University of Lagos, Akoka Lagos, Nigeria Boluwatife Oluwajuwon Owolabi boluwatife.owolabi@aul.edu.ng 10031947532846006549 0009-0008-1696-4249 Yes Department of Anatomy, Faculty of Basic Medical Sciences, University of Lagos, Akoka Lagos, Nigeria Achili Elijah Achili elijahachili23@gmail.com 10031947532846006550 0009-0004-3972-2921 No Department of Anatomy, Faculty of Basic Medical Sciences, University of Lagos, Akoka Lagos, Nigeria Abraham Adewale Adepoju Osinubi aosinubi@unilag.edu.ng 10031947532846006551 10031947532846006551 No Department of Anatomy, Faculty of Basic Medical Sciences, University of Lagos, Akoka Lagos, Nigeria