Journal of Clinical and Basic Research
Journal of Clinical and Basic Research
jcbr
Medical Sciences
http://jcbr.goums.ac.ir
1
admin
2538-3736
10.52547/jcbr
en
jalali
1400
11
1
gregorian
2022
2
1
6
1
online
1
fulltext
en
Evaluation of Plasma Levels of Folic Acid and Homocysteine in Babies Born With Various Types of Neural Tube Defects: A Single Center Study in South India
Biochemistry
پژوهشي
Research
<div style="text-align: justify;"><span style="font-size:10pt"><span new="" roman="" times=""><b><span style="font-size:11.0pt">Background and objectives:</span></b> <span style="font-size:11.0pt">Neural tube defects (NTDs) are common congenital anomalies caused by genetic, environmental, or nutritional factors. Normal plasma folic acid levels in the fetus are required for proper development of the neural tube. Plasma folic acid level has an inverse relationship with homocysteine level. This study aimed to determine plasma folic acid and homocysteine levels in babies born with NTDs and healthy controls.</span><span style="font-size:11.0pt"></span></span></span><br>
<span style="font-size:10pt"><span new="" roman="" times=""><b><span style="font-size:11.0pt">Methods:</span></b> <span style="font-size:11.0pt">The study included 30 clinically diagnosed NTD cases and 30 healthy age- and sex-matched control subjects. Plasma levels of folic acid and homocysteine were measured using a direct chemiluminescence method. Data were compared using the independent t-test. Statistical analysis of data was performed using</span> <span style="font-size:11.0pt">GraphPad InStat 3.0 at significance of 0.05</span><span style="font-size:11.0pt"></span></span></span><br>
<span style="font-size:10pt"><span new="" roman="" times=""><b><span style="font-size:11.0pt">Results:</span></b> <span style="font-size:11.0pt">The mean plasma level of folic acid in NTD cases (5.1±4.9 mol/l) was significantly lower than that in healthy controls (19.5±2.1 mol/l) (<i>p</i><0.05). The mean plasma level of homocysteine in NTD cases (14.3 ± 2.4 ng/ml) was significantly higher than that in healthy controls (4.9±1.8 ng/ml)(<i>p<</i>0.05). The mean plasma level of folic acid was 20.1</span><span style="font-size:11.0pt">±1.5</span><span style="font-size:11.0pt"> µmol/l, 8.5 </span><span style="font-size:11.0pt">±2.9</span><span style="font-size:11.0pt"> µmol/l, and 1.9 </span><span style="font-size:11.0pt">±0.4</span><span style="font-size:11.0pt"> µmol/l in mild, moderate, and severe cases of NTD, respectively. The mean plasma level of homocysteine was 10.7</span><span style="font-size:11.0pt">±3.4</span><span style="font-size:11.0pt"> ng/ml, 15.4</span><span style="font-size:11.0pt">±1.2</span><span style="font-size:11.0pt"> ng/ml, and 18.5</span><span style="font-size:11.0pt">±0.8</span><span style="font-size:11.0pt"> ng/ml in mild, moderate, and severe cases of NTD, respectively.</span><span style="font-size:11.0pt"></span></span></span><br>
<b><span style="font-size:11.0pt"><span new="" roman="" times="">Conclusion:</span></span></b> <span style="font-size:11.0pt"><span new="" roman="" times="">Low level of folic acid and high level of homocysteine are directly associated with the development of neural tube abnormalities. Moreover, the severity of the NTD is inversely related to plasma level of folic acid and directly related to plasma level of homocysteine.</span></span></div>
Neural tube defects, Folic acid, Homocysteine
28
31
http://jcbr.goums.ac.ir/browse.php?a_code=A-10-291-2&slc_lang=en&sid=1
Aravinthan
Singaravelu
arvindtitans99@gmail.com
10031947532846003836
10031947532846003836
No
Department of Anatomy, PSP Medical College, Dr. M.G.R. University, Tamil Nadu, Chennai, India
Swetha
Rajan Vasantharajan
dr.shwetha.rajan@gmail.com
10031947532846003837
10031947532846003837
No
Department of Obstetrics & Gynecology, St.Peter’s Medical College Hospital & Research Institute, Hosur, Tamil Nadu, India
Rajesh
Raveendra
rajesh2361@gmail.com
10031947532846003838
10031947532846003838
No
Department of Anatomy, Phulo Jhano Medical College, Dumka, Jharkhand, India
Anitha
Nancy
ancyjean2010@gmail.com
10031947532846003839
10031947532846003839
Yes
Sri Balaji Vidyapeeth