Hematotoxic ameliorative potential of hydromethanol extract of Justica secunda and astymin in 2, 4-dinitrophenylhydrazine-induced anemic male wistar rats

Mobisson, Samuel Kelechi; Godson, Chiamaka Blossom; Iyanyi, Uchechukwu Loveth; Ehigiator, Ben Enoluomen; Obembe, Agona Odeh

doi:10.61186/jcbr.8.2.10

Volume 8, Issue 2 (Journal of Clinical and Basic Research (JCBR) 2024) jcbr 2024, 8(2): 10-16 | Back to browse issues page

‎ 10.61186/jcbr.8.2.10

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Mobisson S K, Godson C B, Iyanyi U L, Ehigiator B E, Obembe A O. Hematotoxic ameliorative potential of hydromethanol extract of Justica secunda and astymin in 2, 4-dinitrophenylhydrazine-induced anemic male wistar rats. jcbr 2024; 8 (2) :10-16
URL: http://jcbr.goums.ac.ir/article-1-445-en.html

Hematotoxic ameliorative potential of hydromethanol extract of Justica secunda and astymin in 2, 4-dinitrophenylhydrazine-induced anemic male wistar rats

Samuel Kelechi Mobisson¹

, Chiamaka Blossom Godson¹

, Uchechukwu Loveth Iyanyi ^*

², Ben Enoluomen Ehigiator³

, Agona Odeh Obembe⁴

1- Department of Human Physiology, Faculty of Basic Medical Sciences, Madonna University, Elele, Rivers State, Nigeria
2- Department of Pharmacology and Toxicology, Faculty of Pharmacy, Madonna University, Elele, Rivers State, Nigeria , uchechukwuiyanyi@yahoo.com
3- Department of Pharmacology and Therapeutics, Faculty of Basic Clinical Sciences, Edo State University, Uzairue, Nigeria
4- Department of Human Physiology, Faculty of Basic Medical Sciences, University of Calabar, Cross River State, Nigeria

Abstract: (949 Views)

Background: The number of people experiencing a type of anemia in Nigeria is on the rise. Hence, this study examines the comparative impact of Justicia secunda hydro-methanolic leaf extract and astymin on hematologic indices and antioxidant markers in phenylhydrazine (PHZ)-induced anemic male Wister rats.
Methods: Twenty rats weighing between 90g and 170g were divided into five groups (A-E), with four rats in each group. The first group served as control; groups B to E received PHZ, PHZ + astymin (0.5mg/kg), PHZ + J. secunda (0.2mg/kg), and PHZ + J. secunda (0.5mg/kg), respectively. All animals in groups B to E received 2mg/kg PHZ intra-peritoneally for two days, and after that, administration of astymin and J. secunda commenced for 14 days using gavage.
Results: The PCV, RBC, and HB significantly (p<0.01) increased in PHZ + J. secunda treated groups compared to the control. MCHC significantly decreased in PHZ + astymin compared to the control. Total WBC and neutrophil significantly reduced in groups administered with extract compared to control. Monocytes increased significantly in the treated groups compared to the control group. Eosinophils significantly increased in PHZ negative control compared to control. Urea and creatinine increased significantly in the treated groups compared to control. Total Bilirubin increased significantly in groups that received the extract compared to the control group. Conjugated bilirubin was increased significantly in PHZ control and PHZ + astymin compared to control. Total protein significantly increased in PHZ + J. secunda (0.5mg/kg) than control. Lymphocytes, platelets, MCV, MCHC, and albumin concentrations showed no statistically significant alternations. Serum GSH and GPx significantly increased in treated groups compared to control. Catalase significantly (p<0.001) decreased in treated groups than control. Superoxide dismutase significantly increased in the PHZ + astymin group compared to the control group. MDA significantly decreased in the PHZ + astymin group compared to the control group.
Conclusion: PHZ treatment caused toxicity of blood parameters and altered biochemical markers, while astymin and J. secunda treatment had ameliorative effects. Hence, J. secunda may provide benefits in managing anemia and oxidative stress if our findings are confirmed in human subjects.

Keywords: Justicia, Phenylhydrazine, Anemia, Monocytes, Astymin

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Article Type: Research | Subject: Basic medical sciences

References

1. McLean E, Cogswell M, Egli I, Wojdyla D, De-Benoist B. Worldwide prevalence of anemia, WHO vitamin and mineral nutrition information system, 1993-2005. Public Health Nutr. 2009;12(4):444-54. [View at Publisher] [DOI] [PMID] [Google Scholar]

2. Finbarrs BE, Christian EM. and Anikwe VA. Hematological and Histological Effects of Ficus Sycomorus Leaf Extract on Bone Marrow of Phenylhdrazine-Induced Haemolytic Anaemia. J Bio Innov. 2020;9(5):1056-72. [View at Publisher] [DOI] [Google Scholar]

3. Beutler E, Grabowski GA. Gaucher disease: The Metabolic and Molecular Bases of Inherited Disease. 2001:3635-68. [View at Publisher] [Google Scholar]

4. Black MM, Quigg AM, Hurley KM, Pepper MR. Iron deficiency and iron-deficiency anemia in the first two years of life: strategies to prevent loss of developmental potential. Nutr Rev. 2011;69(Suppl1):S64-S70. [View at Publisher] [DOI] [PMID] [Google Scholar]

5. Cappellini MD, Musallam KM, Taher AT. Iron deficiency anaemia revisited. J Intern Med. 2020;287(2):153-70. [View at Publisher] [DOI] [PMID] [Google Scholar]

6. Pandey K, Meena AK, Jain A, Singh RK. Molecular Mechanism of Phenylhydrazine Induced Haematotoxicity: Ame J Phytomed Clin Therapeut. 2014;2(3):390-4. [View at Publisher] [Google Scholar]

7. Hosseini A, Hosseinzadeh H. Effect of Nigella sativa on Blood Diseases: A Review. (PR Victor, RW Ronald, Eds.). Nuts and Seeds in Health and Disease Prevention (2nd Ed.). 2020;315-28. [View at Publisher] [DOI] [Google Scholar]

8. Onochie AU, Helen Oli A, Nnamdi Oli A, Ezeigwe OC, Nwaka AC, Okani CO, et al. The Pharmacobiochemical Effects of Ethanol Extract of Justicia secunda Vahl Leaves in Rattus Norvegicus. J Exp Pharmacol. 2020;12:423-37. [View at Publisher] [DOI] [PMID] [Google Scholar]

9. Fasuyi AO. Nutritional potentials of some tropical vegetable leaf meals: chemical characterization and functional properties. African Journal of Biotechnology. 2006;5(1):49-53. [View at Publisher] [Google Scholar]

10. Sharma AK,Sharma A. Plant secondary metabolites: physicochemical properties and therapeutic applications. Springer. 2022; 371-413. [View at Publisher] [DOI] [Google Scholar]

11. Onyeabo C, Achi NK, Ekeleme-Egedigwe CA, Ebere CU, Okoro CK. Hematological and Biochemical Studies on Justicia Carnea Leaves Extract in Phenylhydrazine Induced-Anemia in Albino Rats. Acta Sci Pol Technol Aliment. 2017;16(2):217-30. [View at Publisher] [DOI] [PMID] [Google Scholar]

12. Umoh RA, Johnny II, Umoh OT, Udoh AE, Anah VU, Obah-Eni LC. Pharmacognostic Evaluation of the Leaves and Stems of Justicia Secunda Vahl. World Journal of Pharmaceutical Research. 2020;9(11):5-18. [View at Publisher] [Google Scholar]

13. Anyasor NG, Azeezat Adenike OA. and Babafemi O. (2019). Evaluation of the anti-inflammatory activity of Justicia secunda Vahl leaf extract using in vitro and in vivo inflammation models. Clinical phytoscience. 2019;5(49):1-13. [View at Publisher] [DOI] [Google Scholar]

14. Onoja SO, Ezeja MI, Omeh YN, Onwukwe BC. Antioxidant, anti-inflammatory, and antinociceptive activities of methanolic extract of Justicia secunda leaves. Alexandria Journal of Medicine. 2017;53(3):207-13. [View at Publisher] [DOI] [Google Scholar]

15. Nneoyi-Egbe AF, Onyenweaku E, Akpanukoh A, Ebai P. Haematinic and hepatoprotective properties of Telfairia occidentalis fruit mesocarp on phenylhydrazine-induced anemia in experimental rats. Biochem Res Int. 2023;2023:8838481. [View at Publisher] [DOI] [PMID] [Google Scholar]

16. Obembe AO, Omini GC, Okon UA, Okpo-ene AI, Ikpi DE. Hematological and immunological effect of Cannabis sativa on Albino Wistar rats. Br J Med Med Res. 2015;7(1):52-60. [View at Publisher] [DOI] [Google Scholar]

17. Mobisson SK, Onyebuagu PC, Wopara I, Woha BJ, Boma HO, Abaka OS, et al. Cannabidiol Oil and Prednisolone Treatment Altered Hematologic Indices, Serum Urea, Creatinine and Cellular Architecture of Kidney on Cadmium Induced Toxicity in Male Wistar Rats. J Pharm Res Int. 2023;35(21):14-27. [View at Publisher] [DOI] [Google Scholar]

18. Ukoh IE, Umoh IB, Ukpai EE, Mobisson SK, Whiskey IP, Azosibe P, et al. Thermoxidized palm oil diet (TPO) induced protein dearangements nin rats is ameliorated by fresh palm oil (FPO) and vitamin E. Journal of Phytopharmacology. 2024;13(2):154-9. [View at Publisher] [DOI] [Google Scholar]

19. Luchese C, Pinton S, Nogueira CW. Brain and lungs of rats are differently affected by cigarette smoke exposure: Antioxidant effect of an organoselenium compound. Pharmacol Res. 2009;59(3):194-201. [View at Publisher] [DOI] [PMID] [Google Scholar]

20. Misra HP, Fridovich I. The role of superoxide anion in the autooxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem. 1972;247(10):3170-5. [View at Publisher] [DOI] [PMID] [Google Scholar]

21. Meenakshi C, Umesh KJ, Mohammed AK, Sunaina Z, Tasneem F. Effect of heavy metal stress on proline, malondialdehyde and superoxide dismutase activity in the cyanobacterium spirulina platensis-S5. Ecotoxicol Environ Saf. 2007;66(2):204-9. [View at Publisher] [DOI] [PMID] [Google Scholar]

22. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by the thiobarbituric acid reaction. Anal Biochem. 1979;95(2):351-8. [View at Publisher] [DOI] [PMID] [Google Scholar]

23. Walker R, Whittlesea C, Eds. Clinical pharmacy and therapeutic. 5th ed. New York:Churchill Livingstone;2007. p.699-701. [View at Publisher]

24. Mpiana PT, Ngbolua KN, Bokota MT, Kasonga TK, Atibu EK, Tshibangu DS, et al. In vitro effects of anthocyanin extracts from Justicia secunda Vahl on the solubility of hemoglobin S and membrane stability of sickle erythrocytes. Blood Transfus. 2010;8(4):248-54. [View at Publisher] [DOI] [PMID] [Google Scholar]

25. Shukla P, Singh RK. Toxicogenomics of Phenylhydrazine Induced Hematotoxicity and its Attenuation by Plumbagin from Plumbago zeylanica. Pharmacogn Mag. 2015;11(Suppl 3):S380-7. [View at Publisher] [DOI] [PMID] [Google Scholar]

26. Mozafari H, Pourpak Z, Pourseyed R, Moein M, Entezari N, Farhadi A, Aghamohammadi A, et al. Health-related quality of life in primary immune deficient patients. Iran J Allergy Asthma Immunol. 2006;5(1):23-7. [View at Publisher] [PMID] [Google Scholar]

27. Guyton AC, Hall JE. Textbook of Medical Physiology. 11th Ed. Philadelphia:Elsevier Saunders;2012. p.802- 4. [View at Publisher] [Google Scholar]

28. Cadman ET, Thysse KA, Bearder S, Cheung AYN, Johnston AC, Lee JJ, et al. Eosinophils Are Important for Protection, Immunoregulation, and Pathology during Infection with Nematode Microfilariae. PloS Pathog. 2014;10(3):e1003988. [View at Publisher] [DOI] [PMID] [Google Scholar]

29. Chaplin DD. Overview of the Immune Response. J Allergy Clin Immunol. 2010;125(Suppl2):S3-23. [View at Publisher] [DOI] [PMID] [Google Scholar]

30. Irinmwinuwa EO, Afonne JO. Investigation into the effect of ethanol leaf extract and fractions of Justicia Secunda on platelets and WBC counts in mice. Magna Scientia Advanced Research and Reviews. 2022;6(1):1-7. [View at Publisher] [DOI] [Google Scholar]

31. Cary R, Dobson S. and Brooke I. Phenylhydrazine. Concise International Chemical Assessment Document 19. 2000:6-27. [View at Publisher]

32. Khatheeja S, Prabhakaran AR, Safiullah A. FTIR-ATR Studies on Phenylhydrazine Induced Hyperbilirubinemia in Wistar Rat. Int J Health Sci Res. 2018;8(2):212-20 [View at Publisher] [Google Scholar]

33. Paul R, Minay J, Christopher C, Damian F, Kelly C. Meta-analysis of the effects of lithium usage on serum creatinine levels. J Psychopharmacol. 2010;24(10):1425-31. [View at Publisher] [DOI] [PMID] [Google Scholar]

34. Jain SK, Hochstein P. Membrane alterations in phenylhydrazine-induced reticulocytes. Arch Biochem Biophys. 1980;201(2):683-7. [View at Publisher] [DOI] [PMID] [Google Scholar]

35. Tejchman K, Kotfis K, Sieńko J. Biomarkers and Mechanisms of Oxidative Stress-Last 20 Years of Research with an Emphasis on Kidney Damage and Renal Transplantation. Int J Mol Sci. 2021;22(15):8010. [View at Publisher] [DOI] [PMID] [Google Scholar]

36. Dalvi U, Naik R, Kale A. Antioxidative enzyme responses against fusarium wilt (fusarium oxysporum f. sp. Ciceris) in chickpea genotypes. Annu Res Rev Biol. 2017;12(5):1-9. [View at Publisher] [DOI] [Google Scholar]

37. Hamilton- Amachree A, Osioma E. Comparative Study on the Phytochemical and in vitro Antioxidant Properties of Methanolic Leaf Extract of Justicia Secunda Vahl. Nigerian Journal of Science and Environment. 2017;15(1):111-7. [View at Publisher] [DOI] [Google Scholar]

38. Mobisson SK, Ikpi DE, Wopara I, Obembe AO. Cannabis sativa exacerbates testicular function by increased oxidative stress, altered male reproductive hormones, sperm quality/quantity, and cellular architecture of the testis. Andrologia. 2022;54(9):e14492. [View at Publisher] [DOI] [PMID] [Google Scholar]

39. Marnett LJ. Oxy radicals, lipid peroxidation, and DNA damage. Toxicology. 2002;181-182:219-22. [View at Publisher] [DOI] [PMID] [Google Scholar]