en Cellular and Molecular Biology پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:200%"><span style="text-autospace:none"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Background and objectives:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""> Cancer stem cells (CSCs) may contribute to tumor initiation, distant metastasis, and chemo-resistance. Quaking (QKI) is a RNA binding protein, a tumor suppressor, and a well-known stem cell biomarker in central nervous system (CNS) cancer. The aim of this study was to identify the potential of QKI mRNA as a prognostic marker for CNS cancer. </span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span style="text-autospace:none"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Methods:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""> The Cancer Genome Atlas (TCGA) was investigated for gene expression profile within CNS cancer data. &nbsp;Further analysis was done through cBioPortal and COSMIC to explore the QKI gene mutation(s). Moreover, QKI mRNA levels were evaluated by using SAGE Genie tools. The Kaplan-Meier Plotter was utilized to identify prognostic role of QKI mRNA levels in these cancers.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span style="text-autospace:none"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Results:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""> Higher levels of QKI mRNA were detected in brain cancer tissues. Altered <i>QKI</i> gene expression was observed in 2% (56/2,958) of patients. Missense <i>QKI</i> gene mutation rate was 35.29%. The <i>QKI</i> gene alterations led to deleterious amino acid changes, including P181R, Q112P, and A220G. Altered <i>QKI</i> gene expression was significantly correlated with reduced survival rate (<i>p</i><0.05). </span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span style="text-autospace:none"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""> The <i>QKI </i>gene is most expressed in brain tissues. In patients with gliomas, altered <i>QKI</i> expression/mutation is associated with a shorter survival rate. The findings of this study indicate that the <i>QKI </i>gene mutations can be considered as a potential prognostic biomarker for brain malignancies.</span></span></span></span></span></span></span><br> &nbsp;</div> Quaking, Biomarker, The Cancer Genome Atlas, neural inflammation disorders 1 11 http://jcbr.goums.ac.ir/browse.php?a_code=A-10-78-1&slc_lang=en&sid=1 Masoumeh Rostami M.rostami@gmail.com 10031947532846003848 10031947532846003848 No Department of Immunotherapy and Vaccine Research, Pasteur Institute of Iran, Tehran, Iran Azam Mirarab azam.mirarab@yahoo.com 10031947532846003849 10031947532846003849 No Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran Alireza Mohebbi Mohebbi-a@goums.ac.ir 10031947532846003850 10031947532846003850 Yes Vista Aria Rena Gene Inc., Golestan Province, Gorgan, Iran