Oluwatoyin Ojuolape Medubi1 
, Leke Jacob Medubi2 , Boluwatife Oluwajuwon Owolabi *3 , Achili Elijah Achili2 , Abraham Adewale Adepoju Osinubi2
, Leke Jacob Medubi2 , Boluwatife Oluwajuwon Owolabi *3 , Achili Elijah Achili2 , Abraham Adewale Adepoju Osinubi2
1- Department of Physiology, Faculty of Basic Medical Sciences, University of Lagos, Akoka Lagos, Nigeria
2- Department of Anatomy, Faculty of Basic Medical Sciences, University of Lagos, Akoka Lagos, Nigeria
3- Department of Anatomy, Faculty of Basic Medical Sciences, University of Lagos, Akoka Lagos, Nigeria ,boluwatife.owolabi@aul.edu.ng
2- Department of Anatomy, Faculty of Basic Medical Sciences, University of Lagos, Akoka Lagos, Nigeria
3- Department of Anatomy, Faculty of Basic Medical Sciences, University of Lagos, Akoka Lagos, Nigeria ,
Abstract: (42 Views)
Background: Spermatogenesis can progress only normally in a tightly regulated redox environment under the fine regulation of testosterone and other hormones. This process is particularly vulnerable to stress, which can disturb the gut microbiome—an important factor in testicular function. However, it is not known if supplementation with probiotics can mitigate the effects of psychological stress on sperm production. This study aimed to investigate the effect of gut probiotics on testicular function of rats during psychological stress.
Methods: Thirty-five adult male rats were randomly assigned into seven groups with five rats each. The research was divided into two phases: one week and two weeks' administration, in which the rats were subjected to probiotics only, stress only, and probiotics plus stress. Data were analyzed using one-way ANOVA and student Newman-Keuls post hoc test for multiple comparisons at p <0.05
Results: Chronically stressed reduced testicular weight and testicular relative organ weight, the same as acutely stressed rats. However, treatment with probiotics resulted in statistically significant differences as a mitigating effect.
Conclusion: Gut probiotics have a generally beneficial impact on the testes when evaluating biochemically and morphological parameters.
Methods: Thirty-five adult male rats were randomly assigned into seven groups with five rats each. The research was divided into two phases: one week and two weeks' administration, in which the rats were subjected to probiotics only, stress only, and probiotics plus stress. Data were analyzed using one-way ANOVA and student Newman-Keuls post hoc test for multiple comparisons at p <0.05
Results: Chronically stressed reduced testicular weight and testicular relative organ weight, the same as acutely stressed rats. However, treatment with probiotics resulted in statistically significant differences as a mitigating effect.
Conclusion: Gut probiotics have a generally beneficial impact on the testes when evaluating biochemically and morphological parameters.
Article Type: Research |
Subject:
Basic medical sciences
References
1. Zhao GQ, Garbers DL. Male germ cell specification and differentiation. Dev Cell . 2002;2(5):537-47. [View at Publisher] [DOI] [PMID] [Google Scholar]
2. Turner Tt. De graaf's thread: the human epididymis.J Androl.2008;29(3):237-50. [View at Publisher] [DOI] [PMID] [Google Scholar]
3. Palomba S, Daolio J, Romeo S, Battaglia FA, Marci R, La Sala GB. Lifestyle and fertility: the influence of stress and quality of life on female fertility. Reprod Biol Endocrinol.2018;16(1):113. [View at Publisher] [DOI] [PMID] [Google Scholar]
4. Ilacqua A, Izzo G, Emerenziani GP, Baldari C, Aversa A. Lifestyle and fertility: the influence of stress and quality of life on male fertility. Reprod Biol Endocrinol . 2018;16(1):115 [View at Publisher] [DOI] [PMID] [Google Scholar]
5. Aitken RJ, Baker MA. The role of genetics and oxidative stress in the etiology of male infertility - a unifying hypothesis?Front Endocrinol (Lausanne)
2020;11:581838. [View at Publisher] [DOI] [PMID] [Google Scholar]
6. Griswold MD. the commitment to meiosis. Physiol Rev. 2016;96(1):1-17. [View at Publisher] [DOI] [PMID] [Google Scholar]
7. Kurkowska W, Bogacz A, Janiszewska M, Gabrys E, Tiszler M, Bellanti F,et Oxidative Stress is Associated with Reduced Sperm Motility in Normal Semen.Am J Mens Health. 2020;14(5):1557988320939731. [View at Publisher] [DOI] [PMID] [Google Scholar]
8. Aitken RJ, Drevet JR. The importance of oxidative stress in determining the functionality of mammalian spermatozoa: A Two-Edged Sword.Antioxidants (Basel). 2020;9(2):111. [View at Publisher] [DOI] [PMID] [Google Scholar]
9. Banihani SA. Role of uric acid in semen. Biomolecules. 2018;8(3):65. [View at Publisher] [DOI] [PMID] [Google Scholar]
10. Rosenberg HF, Masterson JC, Furuta GT. Eosinophils, probiotics, and the microbiome. J Leukoc Biol. 2016;100(5):881-8. [View at Publisher] [DOI] [PMID] [Google Scholar]
11. Baarlen PV, Troosta F, van der Meer C, Hooivelda G, Boekschotena M, Brummera RJM, et al. Human mucosal in vivo transcriptome responses to three lactobacilli indicate how probiotics may modulate human cellular pathways. Proc Natl Acad Sci U S A. 2011;108(Suppl 1):4562-9. [View at Publisher] [DOI] [PMID] [Google Scholar]
12. Konings AW, Drijver EB. Radiation effects on membranes; vitamin E deficiency and lipid peroxidation. Radiat Res. 1979;80(3):494-501. [View at Publisher] [DOI] [PMID] [Google Scholar]
13. Misra HP, Fridovich I. The role of superoxide anion in the autooxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem.
1972;247(10):3170-5. [View at Publisher] [DOI] [PMID] [Google Scholar]
14. Aebi H. Catalase in vitro. Methods Enzymol. 1984;105:121-6. [View at Publisher] [DOI] [PMID] [Google Scholar]
15. Moron MS, Depierre JW, Mannervik B. Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochim Biophys Acta. 1979;582(1):67-78. [View at Publisher] [DOI] [PMID] [Google Scholar]
16. Rostamkhani F, Zardooz H, Zahedias S, Farrokhi B. Comparison of the effects of acute and chronic psychological stress on metabolic features in rats. J Zhejiang Univ Sci B. 2012;13(11):904-12 [View at Publisher] [DOI] [PMID] [Google Scholar]
17. Xiong X, Zhang L, Fan M, Han L, Wu Q , Liu S, et al. β-Endorphin induction by psychological stress promotes leydig cell apoptosis through p38 MAPK pathway in male rats. Cells. 2019;8(10):1265. [View at Publisher] [DOI] [PMID] [Google Scholar]
18. Dong Q, Salva A, Sottas CM, Niu E, Holmes M, Hardy MP. Rapid glucocorticoid mediation of suppressed testosterone biosynthesis in male mice subjected to immobilization stress. J Androl. 2004;25(6):973-81. [View at Publisher] [DOI] [PMID] [Google Scholar]
19. Ribeiro CT, De Souza DB, Costa WS, Sampaio FJ, Pereira Sampaio MA. Immediate and late effects of chronic stress in the testes of prepubertal and adult rats. Asian J Androl. 2018;20(4):385-90. [View at Publisher] [DOI] [PMID] [Google Scholar]
20. Maimaiti Y, Aikebaier A, Maimaitiaili A, Wubulikasimu W, Li YL, Aziguli A, et al. Effects of psychological stress on xanthine oxidase expression, activity and related markers in adipose tissue of mice. Zhongguo Ying Yong Sheng Li Xue Za Zhi . 2019;35(6):537-42. [View at Publisher] [DOI] [PMID] [Google Scholar]
21. Tong S, Zhang P, Cheng Q, Chen M, Chen X, Wang Z, et al. The role of gut microbiota in gout: Is gut microbiota a potential target for gout treatment. Front Cell Infect Microbiol. 2022;12:1051682. [View at Publisher] [DOI] [PMID] [Google Scholar]
22. Lahnsteiner F, Mansour N, Plaetzer K. Antioxidant systems of brown trout (Salmo trutta f. fario) semen. Anim Reprod Sci. 2010;119(3-4):314-21. [View at Publisher] [DOI] [PMID] [Google Scholar]
23. Ma J, Han R, Cui T, Yang C, Wang S. Effects of high serum uric acid levels on oxidative stress levels and semen parameters in male infertile patients. Medicine (Baltimore). 2022;101(3):e28442 [View at Publisher] [DOI] [PMID] [Google Scholar]
24. Stinefelt B, Leonard SS, Blemings KP, Shi X, Klandorf H. Free radical scavenging, DNA protection, and inhibition of lipid peroxidation mediated by uric acid. Ann Clin Lab Sci. 2005;35(1):37-45. [View at Publisher] [PMID] [Google Scholar]
25. Kamat JP. Peroxynitrite: A potent oxidizing and nitrating agent. Indian J Exp Biol. 2006;44(6):436-47. [View at Publisher] [PMID] [Google Scholar]
26. Uribe P, Boguen R, Treulen F, Sanchez R, Villegas JV. Peroxynitrite-mediated nitrosative stress decreases motility and mitochondrial membrane potential in human spermatozoa. Mol Hum Reprod. 2015;21(3):237-43. [View at Publisher] [DOI] [PMID] [Google Scholar]
27. Aitken RJ, Ryan AL, Baker MA, McLaughlin EA. Redox activity associated with the maturation and capacitation of mammalian spermatozoa. Free Radic Biol Med. 2004;36(8):994-1010. [View at Publisher] [DOI] [PMID] [Google Scholar]
28. Liu KS, Mao XD, Pan F, An RF. Effect and mechanisms of reproductive tract infection on oxidative stress parameters, sperm DNA fragmentation, and semen quality in infertile males. Reprod Biol Endocrinol. 2021;19(1):97. [View at Publisher] [DOI] [PMID] [Google Scholar]
29. Huang C, Cao X, Pang D, Li C, Luo Q, Zou Y, et al. Is male infertility associated with increased oxidative stress in seminal plasma? A-meta analysis. Oncotarget. 2018;9(36):24494-513. [View at Publisher] [DOI] [PMID] [Google Scholar]
30. Aitken RJ, Roman SD. Antioxidant systems and oxidative stress in the testes. Oxid Med Cell Longev. 2008:1(1):15-24. [View at Publisher] [DOI] [PMID] [Google Scholar]
31. Medubi LJ, Akinola OB, Oyewopo AO. Low testicular zinc level, p53 expression and impairment of sertoli cell phagocytosis of residual bodies in rat subjected to psychological stress. Andrologia. 2021;53(3):e13958. [View at Publisher] [DOI] [PMID] [Google Scholar]
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