Volume 2, Issue 4 (Journal of Clinical and Basic Research(JCBR) 2018)                   jcbr 2018, 2(4): 48-53 | Back to browse issues page


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1- Department of Biology, Gonbad Kavous University, Gonbad Kavous, Iran
2- Golestan Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Gorgan, Iran
3- *Correspondence: Eisa Jorjani, Department of Biology, Gonbad Kavous Univers
Abstract:   (3635 Views)
Background and objectives: Esophageal cancer (EC) is the second most common gastrointestinal cancer, and esophageal squamous cell carcinoma (ESCC) is the dominant type of EC in Iran. One of the most important challenges in cancer management is the early diagnosis. As tumor suppressors or oncogenes, long non-coding RNAs (lncRNAs) play a vital role in tumor initiation, progression and metastasis. Recent studies have reported dysregulated expression of lncRNAs in different tumor tissues including ESCC, which may indicate its prognostic or diagnostic potential in ESCC. In this study, we investigated expression pattern of lnc-POU3F3 in ESCC.
Methods: We performed quantitative real-time polymerase chain reaction to assess lnc-POU3F3 expression pattern in ESCC blood samples from 32 patients with ESCC and age- and sex-matched healthy controls.
Results: Lnc-POU3F3 was significantly overexpressed in ESCC blood samples compared to normal samples. In addition, this overexpression had a significant correlation with family history (P=0.03) and TNM stage (P=0.02).
Conclusion: According to the findings of the present study, lnc-POU3F3 may be used as a diagnostic biomarker for ESCC. However, studies with a larger sample size should be carried out to confirm this finding.
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Article Type: Research | Subject: Medicine

References
1. Otto T, Sicinski P. Cell cycle proteins as promising targets in cancer therapy. Nature. 2017;17(2):93-115.
2. Malumbres M, Barbacid M. Cell cycle, CDKs and cancer: a changing paradigm. Nature Reviews Cancer. 2009;9(3):153–166. [DOI:10.1038/nrc2602]
3. Hao Y, Wu W, Shi F, Dalmolin RJS, Yan M, Tian F, et al. Prediction of long noncoding RNA functions with co-expression network in esophageal squamous cell carcinoma. BMC Cancer. 2015;15(168):1-10. [DOI:10.1186/s12885-015-1179-z]
4. Wang YL, Bai Y, Yao WJ, Guo L, Wang ZM. Expression of long non coding RNA ZEB1-AS1 in esophageal squamous cell carcinoma and its correlation with tumor progression and patient survival. Int J Clin Exp Pathol. 2015;8(9):11871-11876.
5. Sadjadi A, Nouraie M, Mohagheghi M, MousaviJarrahi A, Malekzadeh R, Donald M. Cancer occurrence in Iran in 2002, an international perspective. Asian Pac J Cancer Prev. 2005;6(3):359-63.
6. Yarhusseini A, Sharifzadeh L, Delpisheh A, Veisani Y, Sayehmiri F, Sayehmir K. Survival Rate of Esophageal Carcinoma in Iran – A Systematic Review and Meta-analysis. Iran J Cancer Prev. 2014;7(2):61-5
7. Li JY, Ma X, Zhang CB. Overexpression of long non-coding RNA UCA1 predicts a poor prognosis in patients with esophageal squamous cell carcinoma. Int J Clin Exp Pathol. 2014;7(11):7938-7944.
8. Gibb EA, Brown CJ Lam WL. The functional role of long non-coding RNA in [DOI:10.1186/1476-4598-10-38]
9. Huttenhofer A, Schattner P, Polacek N. Non-coding RNAs: hope or hype?. Trends in Genetics. 2005;21(5):289-297. [DOI:10.1016/j.tig.2005.03.007]
10. Inamura K. Major Tumor Suppressor and Oncogenic Non-Coding RNAs: Clinical Relevance in Lung Cancer. Cells. 2017;6(2):12-27. [DOI:10.3390/cells6020012]
11. Lv XB, Lian GY, Wang HR, Song E, Yao H, Wang MH. Long noncoding RNA HOTAIR is a prognostic marker for esophageal squamous cell carcinoma progression and survival. Plos One. 2013;8(5):e63516. [DOI:10.1371/journal.pone.0063516]
12. Hu L, Wu Y, Tan D, Meng H, Wang K, Bai Y, et al. Up-regulation of long noncoding RNA MALAT1 contributes to proliferation and metastasis in esophageal squamous cell carcinoma. J. Experimental & Clinical Cancer Research. 2015;34(7):1-12. [DOI:10.1186/s13046-015-0123-z]
13. Xu Y, Wang J, Qiu M, Xu L, Li M, Jiang F, et al. Upregulation of the long noncoding RNA TUG1 promotes proliferation and migration of esophageal squamous cell carcinoma. J. Tumor Biol. 2015;36(3):1643–1651. [DOI:10.1007/s13277-014-2763-6]
14. Tong YS, Wang XW, Zhou XL, Liu ZH, Yang TX, Shi WH, et al. Identification of the long non-coding RNA POU3F3 in plasma as a novel biomarker for diagnosis of esophageal squamous cell carcinoma. J. Molecular Cancer 2015;14(3):1-13. [DOI:10.1186/1476-4598-14-3]
15. Li W, Zheng J, Deng J, You Y, Wu H, Li N, et al. Increased levels of the long intergenic non-protein coding RNA POU3F3 promote DNA methylation in esophageal squamous cell carcinoma cells. Gastroenterology. 2014;146(7):1714-1726. [DOI:10.1053/j.gastro.2014.03.002]
16. Shan TD, Xu JH, Yu T, Li JY, Zhao LN, Ouyang H, et al. Knockdown of linc-POU3F3 suppresses the proliferation, apoptosis, and migration resistance of colorectal cancer. Oncotarget. 2015;7(1):961-975.
17. Guo H, Wu L, Yang Q, Ye M, Zhu X. Functional linc-POU3F3 is overexpressed and contributes to tumorigenesis in glioma. J Gene. 2015;554(1):114-119. [DOI:10.1016/j.gene.2014.10.038]
18. Xiong G, Yang L, Chen Y, Fan Z. Linc-POU3F3 promotes cell proliferation in gastric cancer via increasing T-reg distribution. Am J Transl Res. 2015;7(11):2262-2269.

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