en Genetics پژوهشي Research <p style="color: rgb(0, 0, 0); font-style: normal; font-weight: 400; white-space: normal; -webkit-tap-highlight-color: rgba(26, 26, 26, 0.3); text-decoration: none; line-height: 2.4; text-align: justify;"><span style="font-size:14px;"><span style="font-family:Times New Roman;"><span new="" roman="" times=""><b>Abstract</b></span><br> <span new="" roman="" times=""><b>Background:</b> Cancer treatment using drugs metabolized by the enzymes dihydropyrimidine dehydrogenase (DPYD) and UDP-glucuronosyltransferase 1A1 (UGT1A1) results in adverse effects for some patients. This is frequently reported in cancer patients undergoing therapy with 5-fluorouracil, capecitabine, and irinotecan who have polymorphisms in the genes coding for DPYD and UGT1A1. The present study assessed the DPYD*2A and UGT1A1*28 polymorphisms in cancer patients before starting chemotherapy to identify the individuals at risk of developing an adverse drug reaction.</span><br> <span new="" roman="" times=""><b>Methods:</b> Genomic DNA was isolated from patients and subjected to PCR amplification using specific primers to study DPYD*2A and UGT1A1*28 polymorphisms. The PCR products were assessed by Sanger sequencing for establishing the genotype.</span><br> <span new="" roman="" times=""><b>Results:</b> Of 75 cancer patients requiring treatment with drugs metabolized by DPYD and UGT1A1, 2 (2.66%) and 12 (29.27%) were likely to have adverse reactions based on DPYD*2A and UGT1A1*28 genotyping, respectively.</span><br> <span new="" roman="" times=""><b>Conclusion:</b> Our findings indicate that carrying out genotyping for these two polymorphisms will help a large number of patients requiring treatment with 5-fluorouracil, irinotecan, and capecitabine.</span></span></span></p> Neoplasms,Chemotherapy,Drug therapy,Pharmacogenetics,5-Fluorouracil, Irinotecan,Enzyme 6 8 http://jcbr.goums.ac.ir/browse.php?a_code=A-10-364-1&slc_lang=en&sid=1 Kevin Nischay Gangavarapu kvv9007@gmail.com 10031947532846004288 10031947532846004288 Yes Kamineni Academy of Medical Sciences and Research Center, LB Nagar, Hyderabad, Telangana - 500068, India Nandini Shyamali Bora shyamalinandini@gmail.com 10031947532846004289 10031947532846004289 No Department of Genetics and Molecular Medicine, Kamineni Hospitals, LB Nagar, Hyderabad, Telangana - 500068, India Srinivas Maddali medoncpath@redifmail.com 10031947532846004290 10031947532846004290 No Department of Oncology, Kamineni Hospitals, LB Nagar, Hyderabad, Telangana - 500068, India Qurratulain Hasan qhasan2000@yahoo.com 10031947532846004291 10031947532846004291 No Department of Genetics and Molecular Medicine, Kamineni Hospitals, LB Nagar, Hyderabad, Telangana - 500068, India